Wide-field time-gated SPAD imager for phasor-based FLIM applications

Arin Ulku, Andrei Ardelean, Michel Antolovic, Shimon Weiss, Edoardo Charbon, Claudio Bruschini, Xavier Michalet

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 512 pixels), even in the case of small number of gates and limited photon budget.

Original languageEnglish
Article number024002
JournalMethods and Applications in Fluorescence
Volume8
Issue number2
DOIs
StatePublished - 5 Feb 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 The Author(s). Published by IOP Publishing Ltd.

Keywords

  • FLIM
  • SPAD
  • fluorescence
  • phasor
  • shot noise
  • time-gate
  • wide field

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