Abstract
We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 512 pixels), even in the case of small number of gates and limited photon budget.
Original language | English |
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Article number | 024002 |
Journal | Methods and Applications in Fluorescence |
Volume | 8 |
Issue number | 2 |
DOIs | |
State | Published - 5 Feb 2020 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2020 The Author(s). Published by IOP Publishing Ltd.
Keywords
- FLIM
- SPAD
- fluorescence
- phasor
- shot noise
- time-gate
- wide field