Digital holographic microscopy is the state of the art quantitative phase imaging of micro-objects including living cells. It is an ideal tool to image and quantify cell thickness profiles with nanometer thickness resolution. Digital holographic techniques usually are implemented using a two-beam setup that may be bulky and may not be field portable. Self-referencing techniques provide compact geometry but suffer from a reduction of the field of view. Here, we discuss the development of a wavefront division digital holographic microscope providing the full field of view with a compact system. The proposed approach uses a wavefront division module consisting of two lenses. The developed microscope is tested experimentally by measuring the physical and mechanical properties of red blood cells.
|Number of pages||6|
|Journal||Biomedical Optics Express|
|State||Published - 1 Jun 2018|
Bibliographical noteFunding Information:
Department of Atomic Energy-Board of Research in Nuclear Sciences (DAE-BRNS) (2013/34/11/BRNS/504); Department of Science and Technology-FIST, UGC-DRS.
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.