Wavefront division digital holographic microscopy

Nimit Patel, Vismay Trivedi, Swapnil Mahajan, Vani Chhaniwal, Corinne Fournier, Seonoh Lee, Bahram Javidi, Arun Anand

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Digital holographic microscopy is the state of the art quantitative phase imaging of micro-objects including living cells. It is an ideal tool to image and quantify cell thickness profiles with nanometer thickness resolution. Digital holographic techniques usually are implemented using a two-beam setup that may be bulky and may not be field portable. Self-referencing techniques provide compact geometry but suffer from a reduction of the field of view. Here, we discuss the development of a wavefront division digital holographic microscope providing the full field of view with a compact system. The proposed approach uses a wavefront division module consisting of two lenses. The developed microscope is tested experimentally by measuring the physical and mechanical properties of red blood cells.

Original languageEnglish
Article number#326238
Pages (from-to)2779-2784
Number of pages6
JournalBiomedical Optics Express
Issue number6
StatePublished - 1 Jun 2018
Externally publishedYes

Bibliographical note

Funding Information:
Department of Atomic Energy-Board of Research in Nuclear Sciences (DAE-BRNS) (2013/34/11/BRNS/504); Department of Science and Technology-FIST, UGC-DRS.

Publisher Copyright:
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.


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