The transcriptional process is at the center of the gene expression pathway. In eukaryotes, the transcription of protein-coding genes into messenger RNAs is performed by RNA polymerase II. This enzyme is directed to bind at upstream gene sequences by the aid of transcription factors that assemble transcription-competent complexes. A series of biochemical and structural modifications render the polymerase transcriptionally active so that it can proceed from an initiation state into a functional elongating phase. Recent experimental efforts have attempted to visualize these processes as they take place on genes in living cells and to quantify the kinetics of in vivo transcription.
Bibliographical noteFunding Information:
This work was supported by grants to Yaron Shav-Tal by the Israel Science Foundation, France-Israel Biomedical Imaging program, the Israel Cancer Research Fund and the Israel Cancer Association. Yaron Shav-Tal is the Jane Stern Lebell Family Fellow in Life Sciences at Bar-Ilan University.
- Cellular dynamics
- Live-cell imaging
- mRNA transcription