Viral and host determinants of RNA virus vector replication and expression

Paul Ahlquist; Michael Schwartz; Jianbo Chen; David Kushner; Linhui Hao; Billy T. Dye

doi:10.1016/j.vaccine.2004.11.005

Viral and host determinants of RNA virus vector replication and expression

Paul Ahlquist
, Michael Schwartz
, Jianbo Chen
, David Kushner
, Linhui Hao
, Billy T. Dye

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Positive-strand RNA viruses have proven to be valuable vectors for delivery and expression of antigens for direct vaccination of animals and vaccine production in plants. However, optimal use of these viruses as vectors for vaccine and other purposes is limited by incomplete understanding of their replication pathways and associated constraints on inserted foreign genes. Further insights into RNA virus vector design and optimization are emerging from recent advances on the function of viral RNA replication factors, the nature of the viral RNA replication complex as a membrane-bounded compartment sequestering replication components from competing processes and host defenses, and identification of surprisingly diverse host genes contributing to many virus replication steps.

Original language	English
Pages (from-to)	1784-1787
Number of pages	4
Journal	Vaccine
Volume	23
Issue number	15 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.vaccine.2004.11.005
State	Published - 7 Mar 2005
Externally published	Yes

Bibliographical note

Funding Information:
We thank all members of our laboratory for insights and helpful discussions. This research was supported by the National Institutes of Health through grant GM35072. P.A. is an investigator of the Howard Hughes Medical Institute.

Funding

We thank all members of our laboratory for insights and helpful discussions. This research was supported by the National Institutes of Health through grant GM35072. P.A. is an investigator of the Howard Hughes Medical Institute.

Funders	Funder number
National Institutes of Health
National Institute of General Medical Sciences	R37GM035072

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

RNA virus expression vectors
Vaccine expression
Virus-host interactions

Access to Document

10.1016/j.vaccine.2004.11.005

Cite this

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title = "Viral and host determinants of RNA virus vector replication and expression",

abstract = "Positive-strand RNA viruses have proven to be valuable vectors for delivery and expression of antigens for direct vaccination of animals and vaccine production in plants. However, optimal use of these viruses as vectors for vaccine and other purposes is limited by incomplete understanding of their replication pathways and associated constraints on inserted foreign genes. Further insights into RNA virus vector design and optimization are emerging from recent advances on the function of viral RNA replication factors, the nature of the viral RNA replication complex as a membrane-bounded compartment sequestering replication components from competing processes and host defenses, and identification of surprisingly diverse host genes contributing to many virus replication steps.",

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author = "Paul Ahlquist and Michael Schwartz and Jianbo Chen and David Kushner and Linhui Hao and Dye, \{Billy T.\}",

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AU - Ahlquist, Paul

AU - Schwartz, Michael

AU - Chen, Jianbo

AU - Kushner, David

AU - Hao, Linhui

AU - Dye, Billy T.

N1 - Funding Information: We thank all members of our laboratory for insights and helpful discussions. This research was supported by the National Institutes of Health through grant GM35072. P.A. is an investigator of the Howard Hughes Medical Institute.

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N2 - Positive-strand RNA viruses have proven to be valuable vectors for delivery and expression of antigens for direct vaccination of animals and vaccine production in plants. However, optimal use of these viruses as vectors for vaccine and other purposes is limited by incomplete understanding of their replication pathways and associated constraints on inserted foreign genes. Further insights into RNA virus vector design and optimization are emerging from recent advances on the function of viral RNA replication factors, the nature of the viral RNA replication complex as a membrane-bounded compartment sequestering replication components from competing processes and host defenses, and identification of surprisingly diverse host genes contributing to many virus replication steps.

AB - Positive-strand RNA viruses have proven to be valuable vectors for delivery and expression of antigens for direct vaccination of animals and vaccine production in plants. However, optimal use of these viruses as vectors for vaccine and other purposes is limited by incomplete understanding of their replication pathways and associated constraints on inserted foreign genes. Further insights into RNA virus vector design and optimization are emerging from recent advances on the function of viral RNA replication factors, the nature of the viral RNA replication complex as a membrane-bounded compartment sequestering replication components from competing processes and host defenses, and identification of surprisingly diverse host genes contributing to many virus replication steps.

KW - RNA virus expression vectors

KW - Vaccine expression

KW - Virus-host interactions

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Viral and host determinants of RNA virus vector replication and expression

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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