Vasopressin increases cytosolic free calcium concentration in glomerular mesangial cells

J. V. Bonventre, K. L. Skorecki, J. I. Kreisberg, J. Y. Cheung

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111 Scopus citations


Cytosolic free calcium concentration ([Ca2+](f)) was determined in cultured rat glomerular mesangial cells under basal conditions and after exposure to arginine vasopressin (AVP) or angiotensin II (ANG II). [Ca2+](f) was determined using quin 2 or fura-2, two intracellular fluorescent probes. [Ca2+](f) was measured to be 102 ± 3 nM (n=154) using quin 2 and 82 ± 4 (n=34) using fura-2. AVP and ANG II increased [Ca2+](f). Maximal levels of [Ca2+](f) were achieved in <10 s after addition of the hormone. This peak value was followed by a rapid fall toward the base line. With fura-2 as the intracellular Ca2+ indicator, [Ca2+](f) increased from 74 ± 7 to a peak of 578 ± 39 nM (n=17) with 100 nM AVP. At 115 s after addition of AVP, [Ca2+](f) was 125 ± 9 nM. Similar peak levels of [Ca2+](f) were observed using quin 2. The increase in [Ca2+](f) was due in large part to release of Ca2+ from intracellular stores, since reduction in extracellular free [Ca2+] with EGTA did not prevent the hormone-induced increase in [Ca2+](f), although it did result in a decreased peak level and a more rapid return to the base line. The AVP-induced increase in [Ca2+](f) was blocked by the V1 receptor antagonist (CH2)5Tyr(Me)V(D)AVP. Neither isoproterenol, which increased adenylate cyclase activity, nor dibutyryl cAMP had any affect on [Ca2+](f) directly or on the AVP-induced increase in [Ca2+](f). In this report we present the first direct measurements of [Ca2+](f) and hormone-induced changes in [Ca2+](f) in glomerular mesangial cells. We conclude that AVP increased [Ca2+](f) by interaction with a cellular V1 receptor with subsequent mobilization of intracellular Ca2+ stores. This increase in [Ca2+](f) is likely an intracellular mediator of AVP-induced contraction and prostaglandin production in the mesangial cell.

Original languageEnglish
Pages (from-to)F94-F102
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Issue number1 (20/1)
StatePublished - Jul 1986
Externally publishedYes


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