Validation of different cytotoxicity assays in endothelial cell cultures: Limits in testing cytotoxicity in vitro

K. Peters, S. Stumpf, G. Kamp, H. Schmidt, R. E. Unger, S. Barth, C. J. Kirkpatrick

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Different cytotoxicity assays in human endothelial cells (EC) in vitro were investigated. All agents tested showed concentration-dependent effects in the different assays of cytotoxicity within 24 h except for Cr3+. Although the lactate dehydrogenase-assay (LDH-assay) showed significant cytotoxicity at MD85 concentrations of 1mM, the AlamarBlue assay revealed significant effects beginning with MD85 concentrations of 5 μM. It was suggested that the examination of cell type-specific aspects in testing cytotoxicity could be a further means to increase sensitivity.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages48
Number of pages1
StatePublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Conference

ConferenceTransactions - 7th World Biomaterials Congress
Country/TerritoryAustralia
CitySydney
Period17/05/0421/05/04

Fingerprint

Dive into the research topics of 'Validation of different cytotoxicity assays in endothelial cell cultures: Limits in testing cytotoxicity in vitro'. Together they form a unique fingerprint.

Cite this