UV excited-state photoresponse of Biochromophore negative ions

Anastasia V. Bochenkova, Benedikte Klærke, Dennis B. Rahbek, Jyoti Rajput, Yoni Toker, Lars H. Andersen

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Members of the green fluorescent protein (GFP) family may undergo irreversible phototransformation upon irradiation with UV light. This provides clear evidence for the importance of the higher-energy photophysics of the chromophore, which remains essentially unexplored. By using time-resolved action and photoelectron spectroscopy together with high-level electronic structure theory, we directly probe and identify higher electronically excited singlet states of the isolated para- and meta-chromophore anions of GFP. These molecular resonances are found to serve as a doorway for very efficient electron detachment in the gas phase. Inside the protein, this band is found to be resonant with the quasicontinuum of a solvated electron, thus enhancing electron transfer from the GFP to the solvent. This suggests a photophysical pathway for photoconversion of the protein, where GFP resonant photooxidation in solution triggers radical redox reactions inside these proteins.

Original languageEnglish
Pages (from-to)9797-9801
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number37
DOIs
StatePublished - 8 Sep 2014
Externally publishedYes

Keywords

  • ab initio calculations
  • action spectroscopy
  • electron transfer
  • green fluorescent protein
  • photophysics

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