Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts

Michal Afri; Miriam E. Naqqash; Aryeh A. Frimer

doi:10.1016/j.chemphyslip.2011.09.002

Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts

Michal Afri, Miriam E. Naqqash, Aryeh A. Frimer

Department of Chemistry

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

In previous work, we have shown the utility of the "NMR technique" in locating intercalants within the lipid bilayer. We describe herein the development of a more sensitive and complementary "fluorescence technique" for this purpose and its application to liposomes, bioliposomes and erythrocyte ghosts. This technique is based on the observation in selected compounds of an excellent correlation between the emission wavelength (λ _em) and Dimroth-Reichardt E _T(30) polarity parameter for the solvent in which the fluorescence emission spectrum was obtained.

Original language	English
Pages (from-to)	759-765
Number of pages	7
Journal	Chemistry and Physics of Lipids
Volume	164
Issue number	8
DOIs	https://doi.org/10.1016/j.chemphyslip.2011.09.002
State	Published - Nov 2011

Bibliographical note

Funding Information:
We acknowledge the kind and generous support of The Israel Science Foundation (Grants Number 327/02 and 437/06 ) – founded by The Israel Academy of Sciences and Humanities, and The Ethel and David Resnick Chair in Active Oxygen Chemistry at Bar Ilan University.

Funding

We acknowledge the kind and generous support of The Israel Science Foundation (Grants Number 327/02 and 437/06 ) – founded by The Israel Academy of Sciences and Humanities, and The Ethel and David Resnick Chair in Active Oxygen Chemistry at Bar Ilan University.

Funders	Funder number
Bar-Ilan University
Israel Academy of Sciences and Humanities
Israel Science Foundation	327/02, 437/06

Keywords

E (30)
Erythrocyte ghost
Fluorescence
Intercalation
Liposomes
NMR

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10.1016/j.chemphyslip.2011.09.002

Cite this

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AU - Frimer, Aryeh A.

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AB - In previous work, we have shown the utility of the "NMR technique" in locating intercalants within the lipid bilayer. We describe herein the development of a more sensitive and complementary "fluorescence technique" for this purpose and its application to liposomes, bioliposomes and erythrocyte ghosts. This technique is based on the observation in selected compounds of an excellent correlation between the emission wavelength (λ em) and Dimroth-Reichardt E T(30) polarity parameter for the solvent in which the fluorescence emission spectrum was obtained.

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Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts

Abstract

Bibliographical note

Funding

Keywords

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