Using deep sequencing data for identification of editing sites in mature mirnas

Shahar Alon, Eli Eisenberg

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Deep sequencing has many possible applications; one of them is the identification and quantification of RNA editing sites. The most common type of RNA editing is adenosine to inosine (A-to-I) editing. A prerequisite for this editing process is a double-stranded RNA (dsRNA) structure. Such dsRNAs are formed as part of the microRNA (miRNA) maturation process, and it is therefore expected that miRNAs are affected by A-to-I editing. Indeed, tens of editing sites were found in miRNAs, some of which change the miRNA binding specificity. Here, we describe a protocol for the identification of RNA editing sites in mature miRNAs using deep sequencing data.

Original languageEnglish
Pages (from-to)231-242
Number of pages12
JournalMethods in Molecular Biology
Volume1269
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

  • A-to-I editing
  • Bioinformatics
  • Deep sequencing
  • Genomics
  • MiRNA

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