We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
Bibliographical noteFunding Information:
Funding was provided by EUREKA (R01GM085457) and a Center for Cell Decision Process Grant (P50GM68762) from the US National Institute of Health and by an Institute for Collaborative Biotechnologies Grant (DAAD1903D0004) from the US Army Research Office. M.G. acknowledges support from the Natural Sciences and Engineering Research Council of Canada. F.F.D. acknowledges support from Fundação para a Ciência e a Tecnologia, Portugal, through a graduate fellowship (SFRH/BD/47736/2008).