Uptake of PLGA-Microspheres by human distal lung epithelium and endothelium: A Co-culture model for drug/gene delivery

M. I. Hermanns, S. C. Ma, R. E. Unger, S. Fuchs, W. M. Saltzman, C. J. Kirkpatrick

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The uptake of PLGA-microspheres on human distal lung epithelial cells was studied to examine the novel carrier system for lung drug delivery. Using TEM and SEM, the ultra-structural analysis of adhesion/uptake of spheres to cells was examined. It was observed that incubation with PLGA-microspheres did not effect the trans-bilayer electrical resistance (TER)-values of the co-culture model. It was shown that confocal laser scanning microscopy (CLSM) and TEM demonstrated the localization of microspheres mostly in the cytoplasm.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1814
Number of pages1
StatePublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Conference

ConferenceTransactions - 7th World Biomaterials Congress
Country/TerritoryAustralia
CitySydney
Period17/05/0421/05/04

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