Abstract
Aim: To elucidate the interactions, uptake mechanisms and cytotoxicity profile of glucose-functionalized gold nanoparticles (2GF-GNPs), for expanding and advancing the recently proposed technology of metabolic-based cancer detection to a variety of cancer diseases. Methods: Several cell types with different metabolic features were used to assess the involvement of GLUT-1 and different endocytosis pathways in 2GF-GNP uptake, and the cytotoxicity profile of 2GF-GNPs. Results: Cellular uptake of 2GF-GNP strongly correlated with GLUT-1 surface expression, and occurred mainly through clathrin-mediated endocytosis. 2GF-GNPs showed no toxic effect on cell cycle and proliferation. Conclusion: These findings promote development of metabolic-based cancer detection technologies, and suggest that 2GF-GNPs may enable specific cancer detection in a wide range of tumors characterized by high GLUT-1 expression.
| Original language | English |
|---|---|
| Pages (from-to) | 1535-1549 |
| Number of pages | 15 |
| Journal | Nanomedicine |
| Volume | 13 |
| Issue number | 13 |
| DOIs | |
| State | Published - 2018 |
Bibliographical note
Publisher Copyright:© 2018 2018 Future Medicine Ltd.
Funding
This work was partially supported bythe Israel Science Foundation grant (749/14), by the Israel Cancer Research Fund (ICRF), by the Leon and Maria Taubenblatt Prize for Excellence in Medical Research and by the doctoral scholarship granted to T.D. by the Ministry of Science & Technology, Israel.
| Funders | Funder number |
|---|---|
| Israel Cancer Research Fund | |
| Israel Science Foundation | 749/14 |
| Ministry of science and technology, Israel |
Keywords
- GLUT-1
- cancer
- clathrin-mediated endocytosis
- glucose-coated nanoparticles
- gold nanoparticles
- metabolic-Targeting
