Unilateral renal ischemia reperfusion in the rat: Effect of blood volume trapped in the kidney, sucrose infusion, and antioxidant treatments

Y. Zurovsky, Z. Eligal, S. Grossman

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16 Scopus citations

Abstract

This study was carried out in order to examine whether the severity of acute renal failure observed during the four hours following a 45 min period of unilateral occlusion of the renal pedicle could be reduced by various treatments. These include intrarenal flush with saline immediately before the occlusion, by sucrose infusion immediately before reperfusion, or by injection of NAO (natural antioxidant) and vitamin E before the occlusion. After renal pedicle occlusion, creatinine levels increased to 165 % of their preischemic values. Urine flow, GFR, renal cortex blood flow and NADH decreased by 99 %, 99 %, 50 % and 36 %, respectively. A decrease in the Na and K reabsorption (15 % and 32 %, respectively) was also observed. Partial protection of renal function against ischemic damage was observed when kidney tissue remained blood-free, by exposing it to saline throughout the period of ischemia. Significant protection was observed after treatment with sucrose, vitamin E and NAO. This study demonstrates that it is possible to attenuate the injury to the ischemic kidney by inducing ischemia in a bloodless kidney, by inducing diuresis in the first phase of reperfusion, or by antioxidant treatment, such as vitamin E or NAO.

Original languageEnglish
Pages (from-to)471-478
Number of pages8
JournalExperimental and Toxicologic Pathology
Volume47
Issue number6
DOIs
StatePublished - Dec 1995

Bibliographical note

Funding Information:
Acknowledgements: This study was supported in part by the Health Sciences Research Fund, Yad Hanadiv and the Charles Krown Research Fund, and a grant from the chief scientists of the Ministry of Health, Israel.

Keywords

  • Antioxidants
  • Ischemia, renal
  • Renal failure, acute
  • Renal ischemia
  • Reperfusion, renal ischemia
  • Suerose infusion, renal ischemia
  • Vitamin E, renal ischemia Kidney, ischemia

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