Unified platform for genetic and serological detection of COVID-19 with single-molecule technology

Noa Furth; Shay Shilo; Niv Cohen; Nir Erez; Vadim Fedyuk; Alexander M. Schrager; Adina Weinberger; Amiel A. Dror; Asaf Zigron; Mona Shehadeh; Eyal Sela; Samer Srouji; Sharon Amit; Itzchak Levy; Eran Segal; Rony Dahan; Dan Jones; Daniel C. Douek; Efrat Shema

doi:10.1371/journal.pone.0255096

Unified platform for genetic and serological detection of COVID-19 with single-molecule technology

Noa Furth
, Shay Shilo
, Niv Cohen
, Nir Erez
, Vadim Fedyuk
, Alexander M. Schrager
, Adina Weinberger
, Amiel A. Dror
, Asaf Zigron
, Mona Shehadeh
, Eyal Sela
, Samer Srouji
, Sharon Amit
, Itzchak Levy
, Eran Segal
, Rony Dahan
, Dan Jones
, Daniel C. Douek
, Efrat Shema

Bar-Ilan University - Azrieli Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.

Original language	English
Article number	e0255096
Journal	PLoS ONE
Volume	16
Issue number	7 July
DOIs	https://doi.org/10.1371/journal.pone.0255096
State	Published - Jul 2021

Bibliographical note

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© 2021 Public Library of Science. All rights reserved.

Funding

Funders	Funder number
National Institute of Allergy and Infectious Diseases	ZIAAI005066

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10.1371/journal.pone.0255096

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Furth, N., Shilo, S., Cohen, N., Erez, N., Fedyuk, V., Schrager, A. M., Weinberger, A., Dror, A. A., Zigron, A., Shehadeh, M., Sela, E., Srouji, S., Amit, S., Levy, I., Segal, E., Dahan, R., Jones, D., Douek, D. C., & Shema, E. (2021). Unified platform for genetic and serological detection of COVID-19 with single-molecule technology. PLoS ONE, 16(7 July), Article e0255096. https://doi.org/10.1371/journal.pone.0255096

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abstract = "The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.",

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Furth, N, Shilo, S, Cohen, N, Erez, N, Fedyuk, V, Schrager, AM, Weinberger, A, Dror, AA, Zigron, A, Shehadeh, M, Sela, E, Srouji, S, Amit, S, Levy, I, Segal, E, Dahan, R, Jones, D, Douek, DC & Shema, E 2021, 'Unified platform for genetic and serological detection of COVID-19 with single-molecule technology', PLoS ONE, vol. 16, no. 7 July, e0255096. https://doi.org/10.1371/journal.pone.0255096

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AU - Dror, Amiel A.

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AU - Srouji, Samer

AU - Amit, Sharon

AU - Levy, Itzchak

AU - Segal, Eran

AU - Dahan, Rony

AU - Jones, Dan

AU - Douek, Daniel C.

AU - Shema, Efrat

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Unified platform for genetic and serological detection of COVID-19 with single-molecule technology

Abstract

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