The acclimation of plants to changes in light intensity requires rapid responses at several different levels. These include biochemical and biophysical responses as well as alterations in the steady-state level of different transcripts and proteins. Recent studies utilizing promoter::reporter constructs suggested that transcriptional responses to changes in light intensity could occur within seconds, rates for which changes in mRNA expression are not routinely measured or functionally studied. To identify and characterize rapid changes in the steady-state level of different transcripts in response to light stress we performed RNA sequencing analysis of Arabidopsis thaliana plants subjected to light stress. Here we report that mRNA accumulation of 731 transcripts occurs as early as 20-60 sec following light stress application, and that at least five of these early response transcripts play an important biological role in the acclimation of plants to light stress. More than 20% of transcripts accumulating in plants within 20-60 sec of initiation of light stress are H2O2- and ABA-response transcripts, and the accumulation of several of these transcripts is inhibited by transcriptional inhibitors. In accordance with the association of rapid response transcripts with H2O2 and ABA signaling, a mutant impaired in ABA sensing (abi-1) was found to be more tolerant to light stress, and the response of several of the rapid response transcripts was altered in mutants impaired in reactive oxygen metabolism. Our findings reveal that transcriptome reprogramming in plants could occur within seconds of initiation of abiotic stress and that this response could invoke known as well as unknown proteins and pathways. Significance Statement Transcriptome reprogramming in plants could occur within seconds of abiotic stress initiation and could invoke known as well as unknown proteins and pathways. Here we use light stress as a case study to show that ultra-fast transcriptional responses can reveal important transcripts for abiotic stress acclimation and/or for pathogen resistance.
|Number of pages||13|
|Early online date||26 Sep 2015|
|State||Published - 1 Nov 2015|
Bibliographical noteFunding Information:
This work was supported by funding from the National Science Foundation (IOS-1353886, IOS-0639964, IOS-0743954), the University of North Texas, College of Arts and Sciences, Sophia University, Faculty of Science and Technology, Israel Science Foundation (grant no. 938/11) and Marie Curie Actions-International Career Integration Grant (grant no. 293999). The funders had no role in the design, data collection, analysis, decision to publish or preparation of the manuscript.
© 2015 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
- Arabidopsis thaliana
- light stress