Ubiquitylation-dependent downregulation of Nck regulates its functional activity

Noah Joseph; Barak Reicher; Ahuvit David; Omri Matalon; Mira Barda-Saad

doi:10.1016/j.febslet.2014.08.033

Ubiquitylation-dependent downregulation of Nck regulates its functional activity

Noah Joseph, Barak Reicher, Ahuvit David, Omri Matalon, Mira Barda-Saad

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The Nck adapter protein is involved in key cellular functions, such as actin polymerization and reorganization, serving as a molecular bridge between the surface complex essential for foreign antigen recognition, the T-cell antigen receptor (TCR), and the actin machinery. However, the mechanisms regulating Nck expression and functions are unknown. In this study, we revealed Nck negative regulation and demonstrated that Nck is ubiquitylated following cellular activation. We identified the molecular determinants and mediators involved in this process. Our data suggest that Nck ubiquitylation might serve as a mechanism controlling Nck-mediated effector functions during cellular activation.

Original language	English
Pages (from-to)	3808-3815
Number of pages	8
Journal	FEBS Letters
Volume	588
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2014.08.033
State	Published - 3 Nov 2014

Bibliographical note

Funding Information:
The authors thank Dr. Itay Lazar and Uriel Karo for their technical assistance. This research was funded by the Israel Science Foundation .

Publisher Copyright:
© 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Keywords

Actin machinery
Nck
T-cell signaling
Ubiquitylation

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10.1016/j.febslet.2014.08.033

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abstract = "The Nck adapter protein is involved in key cellular functions, such as actin polymerization and reorganization, serving as a molecular bridge between the surface complex essential for foreign antigen recognition, the T-cell antigen receptor (TCR), and the actin machinery. However, the mechanisms regulating Nck expression and functions are unknown. In this study, we revealed Nck negative regulation and demonstrated that Nck is ubiquitylated following cellular activation. We identified the molecular determinants and mediators involved in this process. Our data suggest that Nck ubiquitylation might serve as a mechanism controlling Nck-mediated effector functions during cellular activation.",

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Ubiquitylation-dependent downregulation of Nck regulates its functional activity

Abstract

Bibliographical note

Keywords

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