UBE2S drives elongation of K11-linked ubiquitin chains by the anaphase-promoting complex

Tao Wu, Yifat Merbl, Ying Huo, Jennifer L. Gallop, Amit Tzur, Marc W. Kirschner

Research output: Contribution to journalArticlepeer-review

186 Scopus citations

Abstract

The Anaphase-Promoting Complex (APC) is an E3 ubiquitin ligase that regulates mitosis and G1 by sequentially targeting cell-cycle regulators for ubiquitination and proteasomal degradation. The mechanism of ubiquitin chain formation by APC and the resultant chain topology remains controversial. By using a single-lysine APC substrate to dissect the topology of ubiquitinated substrates, we find that APC-catalyzed ubiquitination has an intrinsic preference for the K11 linkage of ubiquitin that is essential for substrate degradation. K11 specificity is determined by an E2 enzyme, UBE2S/E2-EPF, that elongates ubiquitin chains after the substrates are pre-ubiquitinated by UbcH10 or UbcH5. UBE2S copurifies with APC; dominant-negative Ube2S slows down APC substrate degradation in functional cell-cycle extracts. We propose that Ube2S is a critical, unique component of the APC ubiquitination pathway.

Original languageEnglish
Pages (from-to)1355-1360
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number4
DOIs
StatePublished - 26 Jan 2010
Externally publishedYes

Keywords

  • Cell cycle
  • E2 enzyme
  • E3 ligase
  • Mitosis
  • Proteasome

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