@article{5ef51359f80d4c24a40825d6c7205c8d,
title = "UBE2S drives elongation of K11-linked ubiquitin chains by the anaphase-promoting complex",
abstract = "The Anaphase-Promoting Complex (APC) is an E3 ubiquitin ligase that regulates mitosis and G1 by sequentially targeting cell-cycle regulators for ubiquitination and proteasomal degradation. The mechanism of ubiquitin chain formation by APC and the resultant chain topology remains controversial. By using a single-lysine APC substrate to dissect the topology of ubiquitinated substrates, we find that APC-catalyzed ubiquitination has an intrinsic preference for the K11 linkage of ubiquitin that is essential for substrate degradation. K11 specificity is determined by an E2 enzyme, UBE2S/E2-EPF, that elongates ubiquitin chains after the substrates are pre-ubiquitinated by UbcH10 or UbcH5. UBE2S copurifies with APC; dominant-negative Ube2S slows down APC substrate degradation in functional cell-cycle extracts. We propose that Ube2S is a critical, unique component of the APC ubiquitination pathway.",
keywords = "Cell cycle, E2 enzyme, E3 ligase, Mitosis, Proteasome",
author = "Tao Wu and Yifat Merbl and Ying Huo and Gallop, {Jennifer L.} and Amit Tzur and Kirschner, {Marc W.}",
year = "2010",
month = jan,
day = "26",
doi = "10.1073/pnas.0912802107",
language = "אנגלית",
volume = "107",
pages = "1355--1360",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "4",
}