Two-photon in vivo imaging of retinal microstructures

Adi Schejter, Nairouz Farah, Shy Shoham

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Non-invasive fluorescence retinal imaging in small animals is an important requirement in an array of translational vision applications. Two-photon imaging has the potential for long-term investigation of healthy and diseased retinal function and structure in vivo. Here, we demonstrate that two-photon microscopy through a mouses pupil can yield high-quality optically sectioned fundus images. By remotely scanning using an electronically tunable lens we acquire highly-resolved 3D fluorescein angiograms. These results provide an important step towards various applications that will benefit from the use of infrared light, including functional imaging of retinal responses to light stimulation.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIV
PublisherSPIE
ISBN (Print)9780819498618
DOIs
StatePublished - 2014
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences XIV - San Francisco, CA, United States
Duration: 2 Feb 20144 Feb 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8948
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIV
Country/TerritoryUnited States
CitySan Francisco, CA
Period2/02/144/02/14

Keywords

  • angiography
  • fundus
  • in-vivo
  • two-photon microscopy

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