Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in E. coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 M(r) protein. The second interferon mRNA species (Hu IFN-β2) is 14S, abou 1300 nucleotides long, and codes for another protein of 23,000-26,000 M(r). The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI x rC), but IFN-β2 mRNA is induced to about 10% in cells by cycloheximide treatment alone, whereas under these conditions IFN-β1 is not induced.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Issue number||12 II|
|State||Published - 1980|