TY - JOUR
T1 - Two interferon mRNAs in human fibroblasts
T2 - In vitro translation and Escherichia coli cloning studies
AU - Weissenbach, J.
AU - Chernajovsky, Y.
AU - Zeevi, M.
AU - Shulman, L.
AU - Soreq, H.
AU - Nir, U.
AU - Wallach, D.
AU - Perricaudet, M.
AU - Tiollais, P.
AU - Revel, M.
PY - 1980/12
Y1 - 1980/12
N2 - Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in E. coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 M(r) protein. The second interferon mRNA species (Hu IFN-β2) is 14S, abou 1300 nucleotides long, and codes for another protein of 23,000-26,000 M(r). The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI x rC), but IFN-β2 mRNA is induced to about 10% in cells by cycloheximide treatment alone, whereas under these conditions IFN-β1 is not induced.
AB - Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in E. coli plasmid pBR322. The major fibroblast interferon (Hu IFN-β1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 M(r) protein. The second interferon mRNA species (Hu IFN-β2) is 14S, abou 1300 nucleotides long, and codes for another protein of 23,000-26,000 M(r). The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI x rC), but IFN-β2 mRNA is induced to about 10% in cells by cycloheximide treatment alone, whereas under these conditions IFN-β1 is not induced.
UR - http://www.scopus.com/inward/record.url?scp=0019128380&partnerID=8YFLogxK
U2 - 10.1073/pnas.77.12.7152
DO - 10.1073/pnas.77.12.7152
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 6164058
AN - SCOPUS:0019128380
SN - 0027-8424
VL - 77
SP - 7152
EP - 7156
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12 II
ER -