TY - JOUR
T1 - Transmission Structured Illumination Microscopy for Quantitative Phase and Scattering Imaging
AU - Wen, Kai
AU - Ma, Ying
AU - Liu, Min
AU - Li, Jianlang
AU - Zalevsky, Zeev
AU - Zheng, Juanjuan
N1 - Publisher Copyright:
© Copyright © 2021 Wen, Ma, Liu, Li, Zalevsky and Zheng.
PY - 2021/1/29
Y1 - 2021/1/29
N2 - In this paper, we demonstrate a digital micromirror device (DMD) based optical microscopic apparatus for quantitative differential phase contrast (qDIC) imaging, coherent structured illumination microscopy (SIM), and dual-modality (scattering/fluorescent) imaging. For both the qDIC imaging and the coherent SIM, two sets of fringe patterns with orthogonal orientations and five phase-shifts for each orientation, are generated by a DMD and projected on a sample. A CCD camera records the generated images in a defocusing manner for qDIC and an in-focus manner for coherent SIM. Both quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed from the recorded intensity images. Moreover, fluorescent imaging modality is integrated, providing specific biochemical structures of the sample once using fluorescent labeling.
AB - In this paper, we demonstrate a digital micromirror device (DMD) based optical microscopic apparatus for quantitative differential phase contrast (qDIC) imaging, coherent structured illumination microscopy (SIM), and dual-modality (scattering/fluorescent) imaging. For both the qDIC imaging and the coherent SIM, two sets of fringe patterns with orthogonal orientations and five phase-shifts for each orientation, are generated by a DMD and projected on a sample. A CCD camera records the generated images in a defocusing manner for qDIC and an in-focus manner for coherent SIM. Both quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed from the recorded intensity images. Moreover, fluorescent imaging modality is integrated, providing specific biochemical structures of the sample once using fluorescent labeling.
KW - multimodality imaging
KW - phase gradient
KW - quantitative phase microscopy
KW - resolution enhancement
KW - structured illumination
UR - http://www.scopus.com/inward/record.url?scp=85100842259&partnerID=8YFLogxK
U2 - 10.3389/fphy.2020.630350
DO - 10.3389/fphy.2020.630350
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AN - SCOPUS:85100842259
SN - 2296-424X
VL - 8
JO - Frontiers in Physics
JF - Frontiers in Physics
M1 - 630350
ER -