Transmission Structured Illumination Microscopy for Quantitative Phase and Scattering Imaging

Kai Wen, Ying Ma, Min Liu, Jianlang Li, Zeev Zalevsky, Juanjuan Zheng

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this paper, we demonstrate a digital micromirror device (DMD) based optical microscopic apparatus for quantitative differential phase contrast (qDIC) imaging, coherent structured illumination microscopy (SIM), and dual-modality (scattering/fluorescent) imaging. For both the qDIC imaging and the coherent SIM, two sets of fringe patterns with orthogonal orientations and five phase-shifts for each orientation, are generated by a DMD and projected on a sample. A CCD camera records the generated images in a defocusing manner for qDIC and an in-focus manner for coherent SIM. Both quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed from the recorded intensity images. Moreover, fluorescent imaging modality is integrated, providing specific biochemical structures of the sample once using fluorescent labeling.

Original languageEnglish
Article number630350
JournalFrontiers in Physics
Volume8
DOIs
StatePublished - 29 Jan 2021

Bibliographical note

Publisher Copyright:
© Copyright © 2021 Wen, Ma, Liu, Li, Zalevsky and Zheng.

Keywords

  • multimodality imaging
  • phase gradient
  • quantitative phase microscopy
  • resolution enhancement
  • structured illumination

Fingerprint

Dive into the research topics of 'Transmission Structured Illumination Microscopy for Quantitative Phase and Scattering Imaging'. Together they form a unique fingerprint.

Cite this