Translocation of acylated pardaxin into cells

Yasmin Paul; Aryeh Weiss; Knut Adermann; Georg Erdmann; Carola Kassebaum; Philip Lazarovici; Jacob Hochman; Hans Wellhöner

doi:10.1016/S0014-5793(98)01389-1

Translocation of acylated pardaxin into cells

Yasmin Paul, Aryeh Weiss, Knut Adermann, Georg Erdmann, Carola Kassebaum, Philip Lazarovici, Jacob Hochman, Hans Wellhöner

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated ¹²⁵I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37-71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated ¹²⁵I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	FEBS Letters
Volume	440
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(98)01389-1
State	Published - 27 Nov 1998
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W.

Funding

This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W.

Funders	Funder number
Lower Saxony-Israel Research Fund

Keywords

Pardaxin uptake
Peptide uptake

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10.1016/S0014-5793(98)01389-1

Cite this

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title = "Translocation of acylated pardaxin into cells",

abstract = "Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated 125I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37-71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated 125I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "Pardaxin uptake, Peptide uptake",

author = "Yasmin Paul and Aryeh Weiss and Knut Adermann and Georg Erdmann and Carola Kassebaum and Philip Lazarovici and Jacob Hochman and Hans Wellh{\"o}ner",

note = "Funding Information: This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W. ",

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AU - Paul, Yasmin

AU - Weiss, Aryeh

AU - Adermann, Knut

AU - Erdmann, Georg

AU - Kassebaum, Carola

AU - Lazarovici, Philip

AU - Hochman, Jacob

AU - Wellhöner, Hans

N1 - Funding Information: This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W.

PY - 1998/11/27

Y1 - 1998/11/27

N2 - Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated 125I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37-71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated 125I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated 125I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37-71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated 125I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - Pardaxin uptake

KW - Peptide uptake

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Translocation of acylated pardaxin into cells

Abstract

Bibliographical note

Funding

Keywords

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