Translational regulation of mast cell USF-2 (fos-interacting protein) synthesis by cytokines and IgE-Ag: The dependency on PKC-β

E. Razin, I. Lewin, Z. C. Zang, V. Kupershtein, J. Jacob-Hirsch, H. Nechushtan, E. Razin

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of c-Fos to bind to various proteins enables the crosstalk between the enzymes that are activated by receptor stimulation and genes that are activated or inactivated as a consequence of a particular stimulus. One of the transcription factors that binds to c-Fos is the USF-2 termed alsoFos-interacting protein. One of the obvious interpretations regarding the intracellular function of USF-2 is that the c-Fos containing AP-1 can be attenuated by the amount of the available Fos as a protein binding panner. Here, we present evidence for the protein kinase C-β (PKC-β) dependent induction of USF-2 protein synthesis and DNA binding activity in murine mast cells activated by either cytokines or by IgE-Ag. Surprisingly, neither one of the stimuli above significantly affected the level of USF-2 mRNA in these cells at any of the time points tested. We concluded that the regulation of USF-2 expression is on the translational rather than on the transcriptional level. Such level of regulation is the first to be described for the expression of early genes. Thus, the synthesis of USF-2 would indirectly control genes whose expression was regulated by AP-1.

Original languageEnglish
Pages (from-to)A1214
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996
Externally publishedYes

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