Transgenic tomato plants expressing the tomato bellow leaf curl virus capsid protein are resistant to the virus

Talya Kunik, Raffi Salomon, Daniel Zamir, Nir Navot, Muhammad Zeidan, Ilana Michelson, Yedidya Gafni, Henryk Czosnek

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

The tomato yellow leaf curl virus (TYLCV) gene that encodes the capsid protein (VI) was placed under transcriptional control of the cauliflower mosaic virus 35S promoter and cloned into an Agrobacterium Ti-derived plasmid and used to transform plants from an interspecific tomato hybrid, Lycopersicon esculentum X L. pennella (Fl), sensitive to the TYLCV disease. When transgenic Fl plants, expressing the VI gene, were inoculated with TYLCV using whiteflies fed on TYLCV-infected plants, they responded either as untransformed tomato or showed expression of delayed disease symptoms and recovery from the disease with increasingly more resistance upon repeated inoculation. Transformed plants that were as sensitive to inoculation as untransformed controls expressed the VI gene at the RNA level only. All the transformed plants that recovered from disease expressed the TYLCV capsid protein.

Original languageEnglish
Pages (from-to)500-504
Number of pages5
JournalBio/Technology
Volume12
Issue number5
DOIs
StatePublished - May 1994
Externally publishedYes

Fingerprint

Dive into the research topics of 'Transgenic tomato plants expressing the tomato bellow leaf curl virus capsid protein are resistant to the virus'. Together they form a unique fingerprint.

Cite this