TY - JOUR
T1 - Transferable resistance factors with mutator effect in Salmonella typhi
AU - Sompolinsky, David
AU - Ben-Yakov, Miriam
AU - Aboud, Mordehai
AU - Boldur, Ida
N1 - Funding Information:
A part of this investigation was supported by a grant 5/E-5 from the Israel Ford Foundation.
PY - 1967
Y1 - 1967
N2 - Strains of Salmonella typhi with different combinations of drug resistance were isolated from samples of feces from one patient. An analysis showed resistance to be caused by two transferable resistance factors, one carrying resistance to chloramphenicol (Cm) and dihydrostreptomycin (Sm), the other to tetracycline (Tc) alone, or to Tc and Sm. Cultures of S. typhi, S. typhimurium and Escherichia coli infected with these resistance factors were genetically unstable, showing high mutability to increased levels of resistance. The high resistance level of the mutants was transferred by conjugation. Plasmid elimination resulted in the loss of this resistance as well as of the high mutability. Results obtained with cultures infected simultaneously with two plasmids indicated that recombination between the Sm loci is possible. The investigation shows that mutations occur on the plasmids, and not as a consequence of interference with the bacterial nucleus.
AB - Strains of Salmonella typhi with different combinations of drug resistance were isolated from samples of feces from one patient. An analysis showed resistance to be caused by two transferable resistance factors, one carrying resistance to chloramphenicol (Cm) and dihydrostreptomycin (Sm), the other to tetracycline (Tc) alone, or to Tc and Sm. Cultures of S. typhi, S. typhimurium and Escherichia coli infected with these resistance factors were genetically unstable, showing high mutability to increased levels of resistance. The high resistance level of the mutants was transferred by conjugation. Plasmid elimination resulted in the loss of this resistance as well as of the high mutability. Results obtained with cultures infected simultaneously with two plasmids indicated that recombination between the Sm loci is possible. The investigation shows that mutations occur on the plasmids, and not as a consequence of interference with the bacterial nucleus.
UR - http://www.scopus.com/inward/record.url?scp=0014061176&partnerID=8YFLogxK
U2 - 10.1016/0027-5107(67)90063-2
DO - 10.1016/0027-5107(67)90063-2
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 5340975
AN - SCOPUS:0014061176
SN - 0027-5107
VL - 4
SP - 119
EP - 127
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 2
ER -