Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of small secreted proteins that promotes biofilm formation and a self-suppression mechanism that prevents their expression. Little is known about the regulatory and structural components of the biofilms in PCC 7942, or response to the suppressor signal(s). We performed transcriptomics (RNA-Seq) and phenomics (RB-TnSeq) screens that identified four genes involved in biofilm formation and regulation, more than 25 additional candidates that may impact biofilm formation, and revealed the transcriptomic adaptation to the biofilm state. In so doing, we compared the effectiveness of these two approaches for gene discovery.
|Journal||Frontiers in Microbiology|
|State||Published - 23 Jun 2022|
Bibliographical noteFunding Information:
Studies were supported by the program of the National Science Foundation and the US-Israel Binational Science Foundation (NSF-BSF 2012823 to RSc and SG). This work was also supported by grants from the Israel Science Foundation (ISF 1406/14 and 2494/19) to RSc.
Copyright © 2022 Simkovsky, Parnasa, Wang, Nagar, Zecharia, Suban, Yegorov, Veltman, Sendersky, Schwarz and Golden.
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