Transcriptomic and Phenomic Investigations Reveal Elements in Biofilm Repression and Formation in the Cyanobacterium Synechococcus elongatus PCC 7942

Ryan Simkovsky, Rami Parnasa, Jingtong Wang, Elad Nagar, Eli Zecharia, Shiran Suban, Yevgeni Yegorov, Boris Veltman, Eleonora Sendersky, Rakefet Schwarz, Susan S. Golden

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of small secreted proteins that promotes biofilm formation and a self-suppression mechanism that prevents their expression. Little is known about the regulatory and structural components of the biofilms in PCC 7942, or response to the suppressor signal(s). We performed transcriptomics (RNA-Seq) and phenomics (RB-TnSeq) screens that identified four genes involved in biofilm formation and regulation, more than 25 additional candidates that may impact biofilm formation, and revealed the transcriptomic adaptation to the biofilm state. In so doing, we compared the effectiveness of these two approaches for gene discovery.

Original languageEnglish
Article number899150
JournalFrontiers in Microbiology
Volume13
DOIs
StatePublished - 23 Jun 2022

Bibliographical note

Publisher Copyright:
Copyright © 2022 Simkovsky, Parnasa, Wang, Nagar, Zecharia, Suban, Yegorov, Veltman, Sendersky, Schwarz and Golden.

Funding

Studies were supported by the program of the National Science Foundation and the US-Israel Binational Science Foundation (NSF-BSF 2012823 to RSc and SG). This work was also supported by grants from the Israel Science Foundation (ISF 1406/14 and 2494/19) to RSc.

FundersFunder number
NSF-BSF2012823
National Science Foundation
Center for Selective C-H Functionalization, National Science Foundation
Center for Hierarchical Manufacturing, National Science Foundation
National Science Foundation, United Arab Emirates
United States-Israel Binational Science Foundation
Israel Science Foundation2494/19, ISF 1406/14
National Science Foundation of Sri Lanka

    Keywords

    • RB-TnSeq
    • RNA-Seq
    • biofilm
    • cyanobacteria
    • transposon library

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