Toward Next-Generation Biohybrid Catalyst Design: Influence of Degree of Polymerization on Enzyme Activity

Marina Kovaliov, Thaiesha A. Wright, Boyle Cheng, Robert T. Mathers, Xiangyu Zhang, Dong Meng, Katarzyna Szcześniak, Jacek Jenczyk, Stefan Jurga, Devora Cohen-Karni, Richard C. Page, Dominik Konkolewicz, Saadyah Averick

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Due to their capacity to conduct complex organic transformations, enzymes find extensive use in medical and industrial settings. Unfortunately, enzymes are limited by their poor stability when exposed to harsh non-native conditions. While a host of methods have been developed to stabilize enzymes in non-native conditions, recent research into the synthesis of polymer-enzyme biohybrids using reversible deactivation radical polymerization approaches has demonstrated the potential of increased enzymatic activity in both native and non-native environments. In this manuscript, we utilize the enzyme lipase, as a model system, to explore the impact that modulation of grafted polymer molecular weight has on enzyme activity in both aqueous and organic media. We studied the properties of these hybrids using both solution-phase enzyme activity methods and coarse-grain modeling to assess the impact of polymer grafting density and grafted polymer molecular weight on enzyme activity to gain a deeper insight into this understudied property of the biohybrid system.

Original languageEnglish
Pages (from-to)939-947
Number of pages9
JournalBioconjugate Chemistry
Issue number3
StatePublished - 18 Mar 2020
Externally publishedYes

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Copyright © 2020 American Chemical Society.


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