Toward dynamic structural biology: Two decades of single-molecule förster resonance energy transfer

Eitan Lerner; Thorben Cordes; Antonino Ingargiola; Yazan Alhadid; Sang Yoon Chung; Xavier Michalet; Shimon Weiss

doi:10.1126/science.aan1133

Toward dynamic structural biology: Two decades of single-molecule förster resonance energy transfer

Eitan Lerner, Thorben Cordes, Antonino Ingargiola, Yazan Alhadid, Sang Yoon Chung, Xavier Michalet, Shimon Weiss

Research output: Contribution to journal › Review article › peer-review

380 Scopus citations

Abstract

Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.

Original language	English
Article number	eaan1133
Journal	Science
Volume	359
Issue number	6373
DOIs	https://doi.org/10.1126/science.aan1133
State	Published - 19 Jan 2018
Externally published	Yes

Bibliographical note

Funding Information:
We acknowledge contributions and discussions with past and present members of the Weiss lab. We thank M. Segal for editing the manuscript. Supported by NIH grants GM069709 and NSF grant MCB-1244175 (S.W.), NIH grant GM095904 (X.M. and S.W.), and European Research Council grant ERC-STG 638536–SM-IMPORT, grant of the Deutsche Forschungsgemeinschaft within GRK2062, the Center for NanoScience, Center for Integrated Protein Science Munich, and LMUexcellent (T.C.).

Funding

We acknowledge contributions and discussions with past and present members of the Weiss lab. We thank M. Segal for editing the manuscript. Supported by NIH grants GM069709 and NSF grant MCB-1244175 (S.W.), NIH grant GM095904 (X.M. and S.W.), and European Research Council grant ERC-STG 638536–SM-IMPORT, grant of the Deutsche Forschungsgemeinschaft within GRK2062, the Center for NanoScience, Center for Integrated Protein Science Munich, and LMUexcellent (T.C.).

Funders	Funder number
LMUexcellent
National Science Foundation	MCB-1244175, GM095904
National Institutes of Health	GM069709
National Institute of General Medical Sciences	R01GM095904
European Commission	ERC-STG 638536–SM-IMPORT
Deutsche Forschungsgemeinschaft	GRK2062
Norsk Sykepleierforbund

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title = "Toward dynamic structural biology: Two decades of single-molecule f{\"o}rster resonance energy transfer",

abstract = "Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule F{\"o}rster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.",

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AU - Chung, Sang Yoon

AU - Michalet, Xavier

AU - Weiss, Shimon

N1 - Funding Information: We acknowledge contributions and discussions with past and present members of the Weiss lab. We thank M. Segal for editing the manuscript. Supported by NIH grants GM069709 and NSF grant MCB-1244175 (S.W.), NIH grant GM095904 (X.M. and S.W.), and European Research Council grant ERC-STG 638536–SM-IMPORT, grant of the Deutsche Forschungsgemeinschaft within GRK2062, the Center for NanoScience, Center for Integrated Protein Science Munich, and LMUexcellent (T.C.).

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Toward dynamic structural biology: Two decades of single-molecule förster resonance energy transfer

Abstract

Bibliographical note

Funding

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