TY - JOUR
T1 - Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions
AU - Barak, Boaz
AU - Williams, Antionette
AU - Bielopolski, Noa
AU - Gottfried, Irit
AU - Okun, Eitan
AU - Brown, Meghan A.
AU - Matti, Ulf
AU - Rettig, Jens
AU - Stuenkel, Edward L.
AU - Ashery, Uri
PY - 2010/12/20
Y1 - 2010/12/20
N2 - The protein tomosyn decreases synaptic transmission and release probability of vesicles, and is essential for modulating synaptic transmission in neurons. In this study, we provide a detailed description of the expression and localization patterns of tomosyn1 and tomosyn2 in the subareas of the mouse hippocampus. Using confocal and two-photon high-resolution microscopy we demonstrate that tomosyn colocalizes with several pre- and postsynaptic markers and is found mainly in glutamatergic synapses. Specifically, we show that tomosyn1 is differentially distributed in the mouse hippocampus and concentrated mainly in the hilus and mossy fibers. Surprisingly, we found that tomosyn2 is expressed in the subiculum, CA1 and CA2 pyramidal cell bodies, dendrites and spines, and colocalizes with PSD95, suggesting a postsynaptic role. These results suggest that in addition to the well-characterized presynaptic function of tomosyn in neurotransmitter release, tomosyn2 might have a postsynaptic function, and place tomosyn as a more general regulator of synaptic transmission and plasticity.
AB - The protein tomosyn decreases synaptic transmission and release probability of vesicles, and is essential for modulating synaptic transmission in neurons. In this study, we provide a detailed description of the expression and localization patterns of tomosyn1 and tomosyn2 in the subareas of the mouse hippocampus. Using confocal and two-photon high-resolution microscopy we demonstrate that tomosyn colocalizes with several pre- and postsynaptic markers and is found mainly in glutamatergic synapses. Specifically, we show that tomosyn1 is differentially distributed in the mouse hippocampus and concentrated mainly in the hilus and mossy fibers. Surprisingly, we found that tomosyn2 is expressed in the subiculum, CA1 and CA2 pyramidal cell bodies, dendrites and spines, and colocalizes with PSD95, suggesting a postsynaptic role. These results suggest that in addition to the well-characterized presynaptic function of tomosyn in neurotransmitter release, tomosyn2 might have a postsynaptic function, and place tomosyn as a more general regulator of synaptic transmission and plasticity.
KW - CA2 area
KW - Hippocampus
KW - Mossy fiber terminals
KW - Synaptic protein
KW - Tomosyn isoforms
UR - http://www.scopus.com/inward/record.url?scp=84862698475&partnerID=8YFLogxK
U2 - 10.3389/fnana.2010.00149
DO - 10.3389/fnana.2010.00149
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C2 - 21191478
AN - SCOPUS:84862698475
SN - 1662-5129
JO - Frontiers in Neuroanatomy
JF - Frontiers in Neuroanatomy
IS - DEC
ER -