Time-gated biological imaging by use of colloidal quantum dots

M. Dahan; T. Laurence; F. Pinaud; D. S. Chemla; A. P. Alivisatos; M. Sauer; S. Weiss

doi:10.1364/ol.26.000825

Time-gated biological imaging by use of colloidal quantum dots

M. Dahan
, T. Laurence
, F. Pinaud
, D. S. Chemla
, A. P. Alivisatos
, M. Sauer
, S. Weiss

Lawrence Berkeley National Laboratory
École normale supérieure
University of California at Berkeley
Heidelberg University

Research output: Contribution to journal › Article › peer-review

337 Scopus citations

Abstract

The long (but not too long) fluorescence lifetime of CdSe semiconductor quantum dots was exploited to enhance fluorescence biological imaging contrast and sensitivity by time-gated detection. Significant and selective reduction of the autofluorescence contribution to the overall image was achieved, and enhancement of the signal-to-background ratio by more than an order of magnitude was demonstrated.

Original language	English
Pages (from-to)	825-827
Number of pages	3
Journal	Optics Letters
Volume	26
Issue number	11
DOIs	https://doi.org/10.1364/ol.26.000825
State	Published - 1 Jun 2001
Externally published	Yes

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10.1364/ol.26.000825

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title = "Time-gated biological imaging by use of colloidal quantum dots",

abstract = "The long (but not too long) fluorescence lifetime of CdSe semiconductor quantum dots was exploited to enhance fluorescence biological imaging contrast and sensitivity by time-gated detection. Significant and selective reduction of the autofluorescence contribution to the overall image was achieved, and enhancement of the signal-to-background ratio by more than an order of magnitude was demonstrated.",

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AU - Dahan, M.

AU - Laurence, T.

AU - Pinaud, F.

AU - Chemla, D. S.

AU - Alivisatos, A. P.

AU - Sauer, M.

AU - Weiss, S.

PY - 2001/6/1

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N2 - The long (but not too long) fluorescence lifetime of CdSe semiconductor quantum dots was exploited to enhance fluorescence biological imaging contrast and sensitivity by time-gated detection. Significant and selective reduction of the autofluorescence contribution to the overall image was achieved, and enhancement of the signal-to-background ratio by more than an order of magnitude was demonstrated.

AB - The long (but not too long) fluorescence lifetime of CdSe semiconductor quantum dots was exploited to enhance fluorescence biological imaging contrast and sensitivity by time-gated detection. Significant and selective reduction of the autofluorescence contribution to the overall image was achieved, and enhancement of the signal-to-background ratio by more than an order of magnitude was demonstrated.

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Time-gated biological imaging by use of colloidal quantum dots

Abstract

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