Thyroid hormones up-regulate Ca-channels in cultured skeletal muscle of the rat

Chaya Brodie, S. R. Sampson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The effects of thyroid hormones (TH) were examined on the expression of slow Ca2+-channels in cultures of rat skeletal muscle. Myotubes were treated with TH at age 5-7 days in vitro, and measurements of specific binding of the dihydropyridine Ca-channel antagonist [3H]PN200-110 were made beginning 12 h later. TH caused a dose-related increase in PN200-110 binding sites with a lower affinity for the ligand than in control cells. The effect was blocked by simultaneous treatment with cycloheximide or actinomycin-D. The results indicate that TH increase gene expression of slow Ca2+-channels of skeletal muscle.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalNeuroscience Letters
Volume117
Issue number3
DOIs
StatePublished - 18 Sep 1990

Keywords

  • Ca-channel
  • Muscle culture
  • Thyroid hormone
  • Triiodothyronine

Fingerprint

Dive into the research topics of 'Thyroid hormones up-regulate Ca-channels in cultured skeletal muscle of the rat'. Together they form a unique fingerprint.

Cite this