Through agarose polyacrolein microsphere polyacrolein microsphere beads-antidigoxin antibodies (apamb-AD)

Shlomo Margel; Leon Marcus; Hillel Savin; Michal Offarim; Aharon Mashiah

doi:10.3109/10731198409118819

Through agarose polyacrolein microsphere polyacrolein microsphere beads-antidigoxin antibodies (apamb-AD)

Shlomo Margel, Leon Marcus, Hillel Savin, Michal Offarim, Aharon Mashiah

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The efficacy of a new, biocompatible, specific immunosorbent for hemoperfusive removal of digoxin is described. The sorbent contains antidigoxin antibodies covalently bound to polyacrolein microspheres, 0.2 μ diam. Thousands of microspheres are matrix-encapsulated in crosslinked agarose to form beads of 500 to 800 μ diam. Digoxin intoxicated dogs showed heart block, ventricular and atrial tachycardia and extended runs of PVCs. During hemoperfusion the abnormal ECG abated; at 2 h of hemoperfusion the ECG tracings returned to normal. Up to 25% of the total digoxin burden was removed. The numbers of the blood cells and the content of a battery of relevant soluble components of the blood remained constant. Dogs were alive and well after the hemoperfusion. Non hemoperfused dogs, which received antiarrythmic agents, did not survive the intoxication. This is the first report of a practical hemoperfusive system for the specific removal of digoxin from whole blood.

Original language	English
Pages (from-to)	25-36
Number of pages	12
Journal	Artificial Cells, Nanomedicine and Biotechnology
Volume	12
Issue number	2 --Jan
DOIs	https://doi.org/10.3109/10731198409118819
State	Published - Jan 1984
Externally published	Yes

Bibliographical note

Funding Information:
We tlinnk Gitan Eshcl, Migada Co, Kiryat Weizmann, Rehovot, Israel, for co111iscl during thc designisg aid fabrication of scvcral prototypes of c;innulil3 2nd )Icrnripcrfuriori outllov arid inllow 3cts mid for providing our l:i[xwat,ory witli IJI 'iry FCLi lor (~valuDiie ri iirltil the currciit models anti Dr. S11aiii ii Gilcr, dircc1,or arid Mi( 1i;icl DaliI, both of tlic lrwt oi Pliysioi and 15 ~ t';liirj "rv IT l J 4 n ,on i'vvl(~Ikn1c h t r. I'cl ah 'l'ilwtli, for gcmcrously prc-vdiiiL space fur thc caw, suigcricj and hclrlopcrluuiuns. 'l'hc rcbcarcd WLS supportcd in part by Galil Advanced Technologies, Ltd., Ramat-Gan, Israel.

Funding

We tlinnk Gitan Eshcl, Migada Co, Kiryat Weizmann, Rehovot, Israel, for co111iscl during thc designisg aid fabrication of scvcral prototypes of c;innulil3 2nd )Icrnripcrfuriori outllov arid inllow 3cts mid for providing our l:i[xwat,ory witli IJI 'iry FCLi lor (~valuDiie ri iirltil the currciit models anti Dr. S11aiii ii Gilcr, dircc1,or arid Mi( 1i;icl DaliI, both of tlic lrwt oi Pliysioi and 15 ~ t';liirj "rv IT l J 4 n ,on i'vvl(~Ikn1c h t r. I'cl ah 'l'ilwtli, for gcmcrously prc-vdiiiL space fur thc caw, suigcricj and hclrlopcrluuiuns. 'l'hc rcbcarcd WLS supportcd in part by Galil Advanced Technologies, Ltd., Ramat-Gan, Israel.

Funders	Funder number
Galil Advanced Technologies, Ltd.

Keywords

APAMB-AD
Antidigoxin antibodies
Beads specific for removal of digoxin
Hemoperfusion
Polyacrolein microspheres
Therapy of digoxin intoxication

Access to Document

10.3109/10731198409118819

Cite this

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title = "Through agarose polyacrolein microsphere polyacrolein microsphere beads-antidigoxin antibodies (apamb-AD)",

abstract = "The efficacy of a new, biocompatible, specific immunosorbent for hemoperfusive removal of digoxin is described. The sorbent contains antidigoxin antibodies covalently bound to polyacrolein microspheres, 0.2 μ diam. Thousands of microspheres are matrix-encapsulated in crosslinked agarose to form beads of 500 to 800 μ diam. Digoxin intoxicated dogs showed heart block, ventricular and atrial tachycardia and extended runs of PVCs. During hemoperfusion the abnormal ECG abated; at 2 h of hemoperfusion the ECG tracings returned to normal. Up to 25% of the total digoxin burden was removed. The numbers of the blood cells and the content of a battery of relevant soluble components of the blood remained constant. Dogs were alive and well after the hemoperfusion. Non hemoperfused dogs, which received antiarrythmic agents, did not survive the intoxication. This is the first report of a practical hemoperfusive system for the specific removal of digoxin from whole blood.",

keywords = "APAMB-AD, Antidigoxin antibodies, Beads specific for removal of digoxin, Hemoperfusion, Polyacrolein microspheres, Therapy of digoxin intoxication",

author = "Shlomo Margel and Leon Marcus and Hillel Savin and Michal Offarim and Aharon Mashiah",

note = "Funding Information: We tlinnk Gitan Eshcl, Migada Co, Kiryat Weizmann, Rehovot, Israel, for co111iscl during thc designisg aid fabrication of scvcral prototypes of c;innulil3 2nd )Icrnripcrfuriori outllov arid inllow 3cts mid for providing our l:i[xwat,ory witli IJI 'iry FCLi lor (~valuDiie ri iirltil the currciit models anti Dr. S11aiii ii Gilcr, dircc1,or arid Mi( 1i;icl DaliI, both of tlic lrwt oi Pliysioi and 15 ~ t';liirj {"}rv IT l J 4 n ,on i'vvl(~Ikn1c h t r. I'cl ah 'l'ilwtli, for gcmcrously prc-vdiiiL space fur thc caw, suigcricj and hclrlopcrluuiuns. 'l'hc rcbcarcd WLS supportcd in part by Galil Advanced Technologies, Ltd., Ramat-Gan, Israel.",

year = "1984",

month = jan,

doi = "10.3109/10731198409118819",

language = "אנגלית",

volume = "12",

pages = "25--36",

journal = "Artificial Cells, Nanomedicine and Biotechnology",

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number = "2 --Jan",

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AU - Marcus, Leon

AU - Savin, Hillel

AU - Offarim, Michal

AU - Mashiah, Aharon

N1 - Funding Information: We tlinnk Gitan Eshcl, Migada Co, Kiryat Weizmann, Rehovot, Israel, for co111iscl during thc designisg aid fabrication of scvcral prototypes of c;innulil3 2nd )Icrnripcrfuriori outllov arid inllow 3cts mid for providing our l:i[xwat,ory witli IJI 'iry FCLi lor (~valuDiie ri iirltil the currciit models anti Dr. S11aiii ii Gilcr, dircc1,or arid Mi( 1i;icl DaliI, both of tlic lrwt oi Pliysioi and 15 ~ t';liirj "rv IT l J 4 n ,on i'vvl(~Ikn1c h t r. I'cl ah 'l'ilwtli, for gcmcrously prc-vdiiiL space fur thc caw, suigcricj and hclrlopcrluuiuns. 'l'hc rcbcarcd WLS supportcd in part by Galil Advanced Technologies, Ltd., Ramat-Gan, Israel.

PY - 1984/1

Y1 - 1984/1

N2 - The efficacy of a new, biocompatible, specific immunosorbent for hemoperfusive removal of digoxin is described. The sorbent contains antidigoxin antibodies covalently bound to polyacrolein microspheres, 0.2 μ diam. Thousands of microspheres are matrix-encapsulated in crosslinked agarose to form beads of 500 to 800 μ diam. Digoxin intoxicated dogs showed heart block, ventricular and atrial tachycardia and extended runs of PVCs. During hemoperfusion the abnormal ECG abated; at 2 h of hemoperfusion the ECG tracings returned to normal. Up to 25% of the total digoxin burden was removed. The numbers of the blood cells and the content of a battery of relevant soluble components of the blood remained constant. Dogs were alive and well after the hemoperfusion. Non hemoperfused dogs, which received antiarrythmic agents, did not survive the intoxication. This is the first report of a practical hemoperfusive system for the specific removal of digoxin from whole blood.

AB - The efficacy of a new, biocompatible, specific immunosorbent for hemoperfusive removal of digoxin is described. The sorbent contains antidigoxin antibodies covalently bound to polyacrolein microspheres, 0.2 μ diam. Thousands of microspheres are matrix-encapsulated in crosslinked agarose to form beads of 500 to 800 μ diam. Digoxin intoxicated dogs showed heart block, ventricular and atrial tachycardia and extended runs of PVCs. During hemoperfusion the abnormal ECG abated; at 2 h of hemoperfusion the ECG tracings returned to normal. Up to 25% of the total digoxin burden was removed. The numbers of the blood cells and the content of a battery of relevant soluble components of the blood remained constant. Dogs were alive and well after the hemoperfusion. Non hemoperfused dogs, which received antiarrythmic agents, did not survive the intoxication. This is the first report of a practical hemoperfusive system for the specific removal of digoxin from whole blood.

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KW - Antidigoxin antibodies

KW - Beads specific for removal of digoxin

KW - Hemoperfusion

KW - Polyacrolein microspheres

KW - Therapy of digoxin intoxication

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SN - 0090-5488

VL - 12

SP - 25

EP - 36

JO - Artificial Cells, Nanomedicine and Biotechnology

JF - Artificial Cells, Nanomedicine and Biotechnology

IS - 2 --Jan

ER -

Through agarose polyacrolein microsphere polyacrolein microsphere beads-antidigoxin antibodies (apamb-AD)

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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