Three-dimensional imaging using phase retrieval with two focus planes

Tali Ilovitsh; Asaf Ilovitsh; Aryeh Weiss; Rinat Meir; Zeev Zalevsky

doi:10.1117/12.2205769

Three-dimensional imaging using phase retrieval with two focus planes

Tali Ilovitsh, Asaf Ilovitsh, Aryeh Weiss, Rinat Meir, Zeev Zalevsky

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

Original language	English
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XXIII
Editors	Carol J. Cogswell, Tony Wilson, Thomas G. Brown
Publisher	SPIE
ISBN (Electronic)	9781628419474
DOIs	https://doi.org/10.1117/12.2205769
State	Published - 2016
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII - San Francisco, United States Duration: 15 Feb 2016 → 17 Feb 2016

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9713
ISSN (Print)	1605-7422

Conference

Conference	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Country/Territory	United States
City	San Francisco
Period	15/02/16 → 17/02/16

Bibliographical note

Publisher Copyright:
© 2016 SPIE.

Keywords

Cellular imaging
Phase retrieval
Three-dimensional microscopy
gold nanoparticle

Access to Document

10.1117/12.2205769

Cite this

Ilovitsh, T., Ilovitsh, A., Weiss, A., Meir, R., & Zalevsky, Z. (2016). Three-dimensional imaging using phase retrieval with two focus planes. In C. J. Cogswell, T. Wilson, & T. G. Brown (Eds.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII Article 97130Y (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9713). SPIE. https://doi.org/10.1117/12.2205769

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abstract = "This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.",

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Ilovitsh, T, Ilovitsh, A, Weiss, A, Meir, R & Zalevsky, Z 2016, Three-dimensional imaging using phase retrieval with two focus planes. in CJ Cogswell, T Wilson & TG Brown (eds), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII., 97130Y, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9713, SPIE, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2205769

Three-dimensional imaging using phase retrieval with two focus planes. / Ilovitsh, Tali; Ilovitsh, Asaf; Weiss, Aryeh et al.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII. ed. / Carol J. Cogswell; Tony Wilson; Thomas G. Brown. SPIE, 2016. 97130Y (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9713).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Ilovitsh T, Ilovitsh A, Weiss A, Meir R, Zalevsky Z. Three-dimensional imaging using phase retrieval with two focus planes. In Cogswell CJ, Wilson T, Brown TG, editors, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII. SPIE. 2016. 97130Y. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2205769

Three-dimensional imaging using phase retrieval with two focus planes

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