Three-dimensional analysis tool for segmenting and measuring the structure of telomeres in mammalian nuclei

Bart J. Vermolen; Ian T. Young; Alice Chuang; London Wark; Tony Chuang; Sabine Mai; Yuval Garini

doi:10.1117/12.590232

Three-dimensional analysis tool for segmenting and measuring the structure of telomeres in mammalian nuclei

Bart J. Vermolen, Ian T. Young, Alice Chuang, London Wark, Tony Chuang, Sabine Mai, Yuval Garini

Research output: Contribution to journal › Conference article › peer-review

Abstract

Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we call ρ_T- This parameter describes if the telomeres are distributed in a sphere-like volume (ρ_T ≈1) or in a disk-like volume (ρ_T ≧1). Because of the statistical nature of this parameter, we have to correct for the fact that we do not have an infinite number of telomeres to calculate this parameter. In this study we show a way to do this correction. After sorting mouse lymphocytes and calculating ρ_T and using the correction introduced in this paper we show a significant difference between nuclei in G2 and nuclei in either G0/G1 or S phase. The mean values of ρ_T for G0/G1, S and G2 are 1.03, 1.02 and 13 respectively.

Original language	English
Article number	17
Pages (from-to)	111-120
Number of pages	10
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5699
DOIs	https://doi.org/10.1117/12.590232
State	Published - 2005
Externally published	Yes
Event	Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States Duration: 24 Jan 2005 → 27 Jan 2005

Keywords

3D imaging
FISH
Fluorescence microscopy
Image processing
Telomeres

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10.1117/12.590232

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title = "Three-dimensional analysis tool for segmenting and measuring the structure of telomeres in mammalian nuclei",

abstract = "Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we call ρT- This parameter describes if the telomeres are distributed in a sphere-like volume (ρT ≈1) or in a disk-like volume (ρT ≧1). Because of the statistical nature of this parameter, we have to correct for the fact that we do not have an infinite number of telomeres to calculate this parameter. In this study we show a way to do this correction. After sorting mouse lymphocytes and calculating ρT and using the correction introduced in this paper we show a significant difference between nuclei in G2 and nuclei in either G0/G1 or S phase. The mean values of ρT for G0/G1, S and G2 are 1.03, 1.02 and 13 respectively.",

keywords = "3D imaging, FISH, Fluorescence microscopy, Image processing, Telomeres",

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year = "2005",

doi = "10.1117/12.590232",

language = "אנגלית",

volume = "5699",

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journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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AU - Vermolen, Bart J.

AU - Young, Ian T.

AU - Chuang, Alice

AU - Wark, London

AU - Chuang, Tony

AU - Mai, Sabine

AU - Garini, Yuval

PY - 2005

Y1 - 2005

N2 - Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we call ρT- This parameter describes if the telomeres are distributed in a sphere-like volume (ρT ≈1) or in a disk-like volume (ρT ≧1). Because of the statistical nature of this parameter, we have to correct for the fact that we do not have an infinite number of telomeres to calculate this parameter. In this study we show a way to do this correction. After sorting mouse lymphocytes and calculating ρT and using the correction introduced in this paper we show a significant difference between nuclei in G2 and nuclei in either G0/G1 or S phase. The mean values of ρT for G0/G1, S and G2 are 1.03, 1.02 and 13 respectively.

AB - Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we call ρT- This parameter describes if the telomeres are distributed in a sphere-like volume (ρT ≈1) or in a disk-like volume (ρT ≧1). Because of the statistical nature of this parameter, we have to correct for the fact that we do not have an infinite number of telomeres to calculate this parameter. In this study we show a way to do this correction. After sorting mouse lymphocytes and calculating ρT and using the correction introduced in this paper we show a significant difference between nuclei in G2 and nuclei in either G0/G1 or S phase. The mean values of ρT for G0/G1, S and G2 are 1.03, 1.02 and 13 respectively.

KW - 3D imaging

KW - FISH

KW - Fluorescence microscopy

KW - Image processing

KW - Telomeres

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T2 - Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

Y2 - 24 January 2005 through 27 January 2005

ER -

Three-dimensional analysis tool for segmenting and measuring the structure of telomeres in mammalian nuclei

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Keywords

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