Abstract
Rat heart cells in culture were found to be a unique model for studying biochemical and pharmacological aspects of thiamine deficiency. When thiamine was excluded from the growth medium, the following effects were observed: (1) Morphological examination did not show any difference between control and thiamine deprived cells during the first 10 days. However, after 10-11 days spontaneous contractions ceased, accompanied by initiation of cell degeneration; (2) Intensive degeneration and cell death were observed after 14-16 days. (3) Thiamine pyrophosphate (TPP) concentration in thiamine-deprived cells was decreased gradually, with an elimination half life of 4-5 days. (4) [3H]deoxyglucose uptake by the cells was increased, even after 1 day of thiamine deprivation. (5) ATP level decreased after 8 days and reached 50% of control cells after 10 days. (6) In thiamine-deprived cells, thiamine addition caused a 60% rise in contraction amplitude but contraction rare was not altered significantly. (7) All these effects were reversible if thiamine was supplied before the initiation of the degeneration processes.
| Original language | English |
|---|---|
| Pages (from-to) | 575-582 |
| Number of pages | 8 |
| Journal | Biochemical Pharmacology |
| Volume | 54 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1 Sep 1997 |
Bibliographical note
Funding Information:We are indebted to Mrs. T. Zinman and A. Isaac for their valuable technical assistance, to S. Victor for typing the mar&&t, to E. Pearl for designing and building the electronic device for contraction mensure-mews, and to Prof. H. Halkin for his goodwill. This research is supported by Grant No. 93-222 f rom the United States-Israel Binational ScLence Foundation (BSF) , J erusalem, Israel, and by the Otto Meyerhoff Drug Receptor Center at Bar-Ilan University.
Funding
We are indebted to Mrs. T. Zinman and A. Isaac for their valuable technical assistance, to S. Victor for typing the mar&&t, to E. Pearl for designing and building the electronic device for contraction mensure-mews, and to Prof. H. Halkin for his goodwill. This research is supported by Grant No. 93-222 f rom the United States-Israel Binational ScLence Foundation (BSF) , J erusalem, Israel, and by the Otto Meyerhoff Drug Receptor Center at Bar-Ilan University.
| Funders | Funder number |
|---|---|
| Otto Meyerhoff Drug Receptor Center at Bar-Ilan University | |
| United States-Israel Binational Science Foundation |
Keywords
- ATP
- Contraction amplitude
- Glucose uptake
- Rat cardiomyocytes
- Thiamine