TY - GEN
T1 - The use of fluorescence fluctuation in polarization sensitive experiments
AU - Fixler, Dror
PY - 2014
Y1 - 2014
N2 - In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.
AB - In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.
KW - Fluorescence
KW - Fluorescence microscopy
KW - Noise in imaging systems
KW - Polarization
KW - Time resolve fluorescence measurements
UR - http://www.scopus.com/inward/record.url?scp=84901790959&partnerID=8YFLogxK
U2 - 10.1117/12.2036490
DO - 10.1117/12.2036490
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AN - SCOPUS:84901790959
SN - 9780819498601
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
PB - SPIE
T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Y2 - 3 February 2014 through 6 February 2014
ER -