The use of fluorescence fluctuation in polarization sensitive experiments

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
PublisherSPIE
ISBN (Print)9780819498601
DOIs
StatePublished - 2014
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII - San Francisco, CA, United States
Duration: 3 Feb 20146 Feb 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8947
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Country/TerritoryUnited States
CitySan Francisco, CA
Period3/02/146/02/14

Keywords

  • Fluorescence
  • Fluorescence microscopy
  • Noise in imaging systems
  • Polarization
  • Time resolve fluorescence measurements

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