The use of fluorescence fluctuation in polarization sensitive experiments

Dror Fixler

doi:10.1117/12.2036490

The use of fluorescence fluctuation in polarization sensitive experiments

Dror Fixler

Bar-Ilan University - The Alexander Kofkin Faculty of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Publisher	SPIE
ISBN (Print)	9780819498601
DOIs	https://doi.org/10.1117/12.2036490
State	Published - 2014
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII - San Francisco, CA, United States Duration: 3 Feb 2014 → 6 Feb 2014

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8947
ISSN (Print)	1605-7422

Conference

Conference	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Country/Territory	United States
City	San Francisco, CA
Period	3/02/14 → 6/02/14

Keywords

Fluorescence
Fluorescence microscopy
Noise in imaging systems
Polarization
Time resolve fluorescence measurements

Access to Document

10.1117/12.2036490

Cite this

@inproceedings{a626ae2d5a754607a25351822121bc25,

title = "The use of fluorescence fluctuation in polarization sensitive experiments",

abstract = "In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.",

keywords = "Fluorescence, Fluorescence microscopy, Noise in imaging systems, Polarization, Time resolve fluorescence measurements",

author = "Dror Fixler",

year = "2014",

doi = "10.1117/12.2036490",

language = "אנגלית",

isbn = "9780819498601",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII",

address = "ארצות הברית",

note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII ; Conference date: 03-02-2014 Through 06-02-2014",

}

Fixler, D 2014, The use of fluorescence fluctuation in polarization sensitive experiments. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII., 894717, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8947, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, San Francisco, CA, United States, 3/02/14. https://doi.org/10.1117/12.2036490

TY - GEN

T1 - The use of fluorescence fluctuation in polarization sensitive experiments

AU - Fixler, Dror

PY - 2014

Y1 - 2014

N2 - In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.

AB - In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. A new method to extract the rotational correlation time of molecule in fluorescence fluctuation polarization sensitive measurements is suggested in our talk. This new method is advantageous in cases where the rotational correlation time of the fluorescent molecule is much lower than the temporal resolution of the system, or in cases where the rotational correlation time is not observed by standard autocorrelation analysis methods such as fluorescence correlation spectroscopy (FCS) or rotational correlation functions analysis.

KW - Fluorescence

KW - Fluorescence microscopy

KW - Noise in imaging systems

KW - Polarization

KW - Time resolve fluorescence measurements

UR - http://www.scopus.com/inward/record.url?scp=84901790959&partnerID=8YFLogxK

U2 - 10.1117/12.2036490

DO - 10.1117/12.2036490

M3 - ???researchoutput.researchoutputtypes.contributiontobookanthology.conference???

AN - SCOPUS:84901790959

SN - 9780819498601

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

PB - SPIE

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

Y2 - 3 February 2014 through 6 February 2014

ER -

The use of fluorescence fluctuation in polarization sensitive experiments

Abstract

Publication series

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this