The tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1

Amalia Bar-Ziv; Yael Levy; Hagit Hak; Anahit Mett; Eduard Belausov; Vitaly Citovsky; Yedidya Gafni

doi:10.4161/psb.20935

The tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1

Amalia Bar-Ziv, Yael Levy, Hagit Hak, Anahit Mett, Eduard Belausov, Vitaly Citovsky, Yedidya Gafni

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The V2 protein of Tomato yellow leaf curl geminivirus (TYLCV) is an RNA-silencing suppressor that counteracts the innate immune response of the host plant. However, this anti-host defense function of V2 may include targeting of other defensive mechanisms of the plant. Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. This binding occurred both in vitro and in vivo, within living plant cells. The V2 binding site within mCYP1 was identified in the direct proximity to the papain-like cysteine protease active site.

Original language	English
Pages (from-to)	983-989
Number of pages	7
Journal	Plant Signaling and Behavior
Volume	7
Issue number	8
DOIs	https://doi.org/10.4161/psb.20935
State	Published - Aug 2012

Bibliographical note

Funding Information:
This work was supported by the US-Israel Binational Agricultural Research and Development (BARD) grant IS-4237 to V.C. and Y.G., and by the US-Israel Binational Science Foundation (BSF) grant 2007140 to Y.G. The work in V.C.’s lab was supported by grants from NIH, NSF and NIFA/USDA. This paper is a contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 106/2012

Funding

This work was supported by the US-Israel Binational Agricultural Research and Development (BARD) grant IS-4237 to V.C. and Y.G., and by the US-Israel Binational Science Foundation (BSF) grant 2007140 to Y.G. The work in V.C.’s lab was supported by grants from NIH, NSF and NIFA/USDA. This paper is a contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 106/2012

Funders	Funder number
National Institute of General Medical Sciences	R01GM050224
United States - Israel Binational Agricultural Research and Development Fund	IS-4237
Agricultural Research Council	106/2012
United States-Israel Binational Science Foundation	2007140

Keywords

CYP1
Papain-like cysteine protease
Protein interaction
TYLCV
V2 protein

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10.4161/psb.20935

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abstract = "The V2 protein of Tomato yellow leaf curl geminivirus (TYLCV) is an RNA-silencing suppressor that counteracts the innate immune response of the host plant. However, this anti-host defense function of V2 may include targeting of other defensive mechanisms of the plant. Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. This binding occurred both in vitro and in vivo, within living plant cells. The V2 binding site within mCYP1 was identified in the direct proximity to the papain-like cysteine protease active site.",

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AU - Belausov, Eduard

AU - Citovsky, Vitaly

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The tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1

Abstract

Bibliographical note

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Keywords

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