The Tomato yellow leaf curl virus (TYLCV) V2 protein inhibits enzymatic activity of the host papain-like cysteine protease CYP1

Amalia Bar-Ziv; Yael Levy; Vitaly Citovsky; Yedidya Gafni

doi:10.1016/j.bbrc.2015.03.063

The Tomato yellow leaf curl virus (TYLCV) V2 protein inhibits enzymatic activity of the host papain-like cysteine protease CYP1

Amalia Bar-Ziv
, Yael Levy
, Vitaly Citovsky
, Yedidya Gafni

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The viral V2 protein is one of the key factors that Tomato yellow leaf curl geminivirus (TYLCV), a major tomato pathogen worldwide, utilizes to combat the host defense. Besides suppressing the plant RNA silencing defense by targeting the host SGS3 component of the silencing machinery, V2 also interacts with the host CYP1 protein, a papain-like cysteine protease likely involved in hypersensitive response reactions. The biological effects of the V2-CYP1 interaction, however, remain unknown. We addressed this question by demonstrating that V2 inhibits the enzymatic activity of CYP1, but does not interfere with post-translational maturation of this protein.

Original language	English
Pages (from-to)	525-529
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	460
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2015.03.063
State	Published - 8 May 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.

Funding

This work was supported by the US–Israel Binational Agricultural Research and Development (BARD) grant IS-4605-13C and by the US–Israel Binational Science Foundation (BSF) grant 2011070 , to V.C. and Y.G. V.C. is also supported by NIH , grant R01 GM50224 , USDA/NIFA , grant 2013-02918 and NSF , grant MCB1118491 . This paper is a contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 216/2014.

Funders	Funder number
USDA/NIFA	2013-02918
US–Israel Binational Agricultural Research and Development
US-Israel Binational Science Foundation
National Science Foundation	MCB1118491
National Institutes of Health
National Institute of General Medical Sciences	R01GM050224
United States - Israel Binational Agricultural Research and Development Fund	IS-4605-13C
United States-Israel Binational Science Foundation	2011070

Keywords

CYP1
Papain-like cysteine protease
Programmed cell death
TYLCV
V2 protein

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10.1016/j.bbrc.2015.03.063

Cite this

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title = "The Tomato yellow leaf curl virus (TYLCV) V2 protein inhibits enzymatic activity of the host papain-like cysteine protease CYP1",

abstract = "The viral V2 protein is one of the key factors that Tomato yellow leaf curl geminivirus (TYLCV), a major tomato pathogen worldwide, utilizes to combat the host defense. Besides suppressing the plant RNA silencing defense by targeting the host SGS3 component of the silencing machinery, V2 also interacts with the host CYP1 protein, a papain-like cysteine protease likely involved in hypersensitive response reactions. The biological effects of the V2-CYP1 interaction, however, remain unknown. We addressed this question by demonstrating that V2 inhibits the enzymatic activity of CYP1, but does not interfere with post-translational maturation of this protein.",

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AB - The viral V2 protein is one of the key factors that Tomato yellow leaf curl geminivirus (TYLCV), a major tomato pathogen worldwide, utilizes to combat the host defense. Besides suppressing the plant RNA silencing defense by targeting the host SGS3 component of the silencing machinery, V2 also interacts with the host CYP1 protein, a papain-like cysteine protease likely involved in hypersensitive response reactions. The biological effects of the V2-CYP1 interaction, however, remain unknown. We addressed this question by demonstrating that V2 inhibits the enzymatic activity of CYP1, but does not interfere with post-translational maturation of this protein.

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The Tomato yellow leaf curl virus (TYLCV) V2 protein inhibits enzymatic activity of the host papain-like cysteine protease CYP1

Abstract

Bibliographical note

Funding

Keywords

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