The support-on-support concept for in-situ oligonucleotide synthesis on nanoparticles

H. Seliger, M. Hinz, R. Ditz, M. Koch, P. Lapido, S. Margel

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Oligonucleotide-loaded nanoparticles, which are of interest for biomedical application, up to now, could not be prepared by in-situ synthesis, due to difficulty of handling in automated synthesizers. To overcome this problem, we have introduced the "support-on-support" concept. It is based on the reversible anchoring of nanoparticles to the surface of microparticles. These composite beads easily can be used for automated synthesis, being released after completion of chain elongations. As examples, dextran-coated magnetite nanoparticles were attached to polystyrene microparticles through (1) a gelatine or (2) a silica layer. Release involved dissolution of the bonding layer by (1) proteases or (2) alkali.

Original languageEnglish
Pages (from-to)1167-1172
Number of pages6
JournalNucleosides, Nucleotides and Nucleic Acids
Issue number8-9
StatePublished - Aug 2007

Bibliographical note

Funding Information:
Financial support by the German Bundesministerium für Forschung und Technologie is gratefully acknowledged. The authors thank C. Egger for technical assistance. Address correspondence to H. Seliger, University of Ulm, Arbeitsgruppe Chemische Funktionen in Biosystemem, D-89069, Germany. E-mail:


  • In situ synthesis
  • Nanoparticles
  • Oligonucleotide
  • Support-on-support concept


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