The role of Nitric oxide in the ischemic brain evaluated by spectroscopic monitoring of mitochondrial nadh, microcirculatory blood flow and HbO 2

Eibeshitz Efrat, Barbiro Michaely Efrat, Mayevsky Avraham

Research output: Contribution to journalConference articlepeer-review

Abstract

Nitric oxide (NO) is involved in the autoregulation of cerebral blood flow and in modulation of the respiratory chain activity in the normal brain. The present study aimed to evaluate the role of NO under two levels of partial brain ischemia induced by unilateral or bilateral carotid artery occlusion (UCO/BCO), for 24 hours and in appropriate control groups. Time-sharing fluorometer/reflectometer was used for the monitoring of mitochondrial NADH, by the fluorometric technique and oxyhemoglobin level by 2 wavelength reflectance method, combined with laser Doppler flowmetry for cerebral blood flow (CBF) monitoring. Systemic blood pressure (MAP was also monitored. L-NAME was used for the inhibition of NO synthesis while nitrite was used as an exogenous source for NO. In both groups of ischemic animals L-NAME induced an increase in MAP and oxigenation of HbO2 however in the UCO group CBF remained stable and NADH was oxidized whereas in the BCO group CBF decreased with no change in NADH. Following nitrite infusion MAP reversibly decreased, CBF increased, HbO2 and NADH remained low in the BCO group with a slight reversible increase of NADH in the UCO group .In conclusion, it seems that NO plays an important role in the autoregulation of CBF and mitochondrial activity in the partially ischemic brain.

Original languageEnglish
Article number72801J
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7280
DOIs
StatePublished - 2009
Event7th International Conference on Photonics and Imaging in Biology and Medicine - Wuhan, China
Duration: 24 Nov 200827 Nov 2008

Keywords

  • Cerebral blood flow
  • Mitochondrial function
  • Nitric oxide
  • Tissue oxygenation

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