TY - JOUR
T1 - The relationship between sperm ultrastructural features and fertilizing capacity in vitro
AU - Mashiach, R.
AU - Fisch, B.
AU - Eltes, F.
AU - Tadir, Y.
AU - Ovadia, J.
AU - Bartoov, B.
PY - 1992/5
Y1 - 1992/5
N2 - Objective: To evaluate the relationship between ultramorphological features of the human sperm and its fertilizing capacity in vitro. Design: The study was performed retrospectively. Ultrastructural features were assessed using scanning and transmission electron microscopes in sperm samples of individuals who underwent an in vitro fertilization (IVF) treatment cycle no more than 6 months before the study. Setting: Institutional clinical care. Patients: Fifty-six infertile couples in whom mechanical infertility was diagnosed in the female partner. Patients were categorized as fertilizing when fertilization of at least 30% of the oocytes occurred (n = 27) and nonfertilizing when none of the oocytes fertilized in at least two consecutive IVF treatment cycles (n = 29). Results: The two groups differed significantly only in ultramorphological parameters of the sperm head and acrosome (head, F(8,36) = 2.8, P < 0.02; acrosome, F(4,40) = 2.8, P < 0.04), and especially in the following malformation patterns: hyperelongated head, acrosome deficiency, and acrosome damage. The suggested score based on these findings was able to predict 90% and 76% of the cases with and without fertilizing potential, respectively. Conclusions: The ultrastructural morphology of the sperm head components is a key parameter for assessing the sperm fertilizing capacity in vitro.
AB - Objective: To evaluate the relationship between ultramorphological features of the human sperm and its fertilizing capacity in vitro. Design: The study was performed retrospectively. Ultrastructural features were assessed using scanning and transmission electron microscopes in sperm samples of individuals who underwent an in vitro fertilization (IVF) treatment cycle no more than 6 months before the study. Setting: Institutional clinical care. Patients: Fifty-six infertile couples in whom mechanical infertility was diagnosed in the female partner. Patients were categorized as fertilizing when fertilization of at least 30% of the oocytes occurred (n = 27) and nonfertilizing when none of the oocytes fertilized in at least two consecutive IVF treatment cycles (n = 29). Results: The two groups differed significantly only in ultramorphological parameters of the sperm head and acrosome (head, F(8,36) = 2.8, P < 0.02; acrosome, F(4,40) = 2.8, P < 0.04), and especially in the following malformation patterns: hyperelongated head, acrosome deficiency, and acrosome damage. The suggested score based on these findings was able to predict 90% and 76% of the cases with and without fertilizing potential, respectively. Conclusions: The ultrastructural morphology of the sperm head components is a key parameter for assessing the sperm fertilizing capacity in vitro.
KW - Sperm ultramorphology
KW - fertilizing capacity
KW - in vitro fertilization
UR - http://www.scopus.com/inward/record.url?scp=0026763996&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(16)55024-9
DO - 10.1016/S0015-0282(16)55024-9
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C2 - 1572473
AN - SCOPUS:0026763996
SN - 0015-0282
VL - 57
SP - 1052
EP - 1057
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 5
ER -