The power and prospects of fluorescence microscopies and spectroscopies

Xavier Michalet, Achillefs N. Kapanidis, Ted Laurence, Fabien Pinaud, Soeren Doose, Malte Pflughoefft, Shimon Weiss

Research output: Contribution to journalReview articlepeer-review

188 Scopus citations


Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy.

Original languageEnglish
Pages (from-to)161-182
Number of pages22
JournalAnnual Review of Biophysics and Biomolecular Structure
StatePublished - 2003
Externally publishedYes


  • FRET
  • Lifetime
  • Polarization
  • Single-molecule
  • Two-photon


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