The pK of Schiff base deprotonation in bacteriorhodopsin

Benjamin Ehrenberg, Aaron Lewis

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Kinetic resonance Raman spectroscopy as a function of pH has been utilized to determine the pK of Schiff base deprotonation during the bacteriorhodopsin photochemical cycle. It is shown that the pK of Schiff base deprotonation is between 9.9 and 10.3, microseconds after light absorption and is >12 before photon initiation of photochemical cycling associated with proton pumping.

Original languageEnglish
Pages (from-to)1154-1159
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume82
Issue number4
DOIs
StatePublished - 29 Jun 1978
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements. This work was supported Institutes of Health

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