The nucleolar PICT-1/GLTSCR2 protein forms homo-oligomers

Tatyana Borodianskiy-Shteinberg; Inna Kalt; Sarit Kipper; Nofar Nachum; Shiri Katz; Maor H. Pauker; Mira Barda-Saad; Doron Gerber; Ronit Sarid

doi:10.1016/j.jmb.2014.04.006

The nucleolar PICT-1/GLTSCR2 protein forms homo-oligomers

Tatyana Borodianskiy-Shteinberg, Inna Kalt, Sarit Kipper, Nofar Nachum, Shiri Katz, Maor H. Pauker, Mira Barda-Saad, Doron Gerber, Ronit Sarid

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The human "protein interacting with carboxyl terminus 1" (PICT-1), also designated as the "glioma tumor suppressor candidate region 2 gene product", GLTSCR2, is a nucleolar protein whose activity is, as yet, unknown. Contradictory results regarding the role of PICT-1 in cancer have been reported, and PICT-1 has been suggested to function either as a tumor suppressor protein or as an oncogene. In this study, we demonstrate self-association of PICT-1. Through yeast two-hybrid assay, we identified PICT-1 as its own interaction partner. We confirmed the interaction of PICT-1 with itself by direct yeast two-hybrid assay and also showed self-association of PICT-1 in mammalian cells by co-immunoprecipitation and fluorescence resonance energy transfer assays. Furthermore, we confirmed direct self-association of PICT-1 by using in vitro microfluidic affinity binding assays. The later assay also identified the carboxy-terminal domain as mediating self-interaction of PICT-1. Glutaraldehyde cross-linking and gel-filtration assays suggest that PICT-1 forms dimers, though it may form higher-order complexes as well. Our findings add another layer of complexity in understanding the different functions of PICT-1 and may help provide insights regarding the activities of this protein.

Original language	English
Pages (from-to)	2363-2378
Number of pages	16
Journal	Journal of Molecular Biology
Volume	426
Issue number	12
DOIs	https://doi.org/10.1016/j.jmb.2014.04.006
State	Published - 12 Jun 2014

Bibliographical note

Funding Information:
We gratefully acknowledge Prof. Bernard Roizman, Dr. Jordan Chill and Dr. Yarden Opatowsky for reagents, helpful discussions and technical help. Portions of this work submitted by T.B.-S. to Bar Ilan University, Ramat Gan, Israel, are in partial fulfillment of the requirements for the degree of Doctor of Philosophy. The anti-myc developed by Prof. J. M. Bishop was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by The University of Iowa, Department of Biology, Iowa City, IA, USA. This work was supported by grants from the Israel Cancer Association , the Chief Scientist's Office of the Ministry of Health, Israel , and the Israel Science Foundation (Grant No. 709/10 ).

Funding

We gratefully acknowledge Prof. Bernard Roizman, Dr. Jordan Chill and Dr. Yarden Opatowsky for reagents, helpful discussions and technical help. Portions of this work submitted by T.B.-S. to Bar Ilan University, Ramat Gan, Israel, are in partial fulfillment of the requirements for the degree of Doctor of Philosophy. The anti-myc developed by Prof. J. M. Bishop was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by The University of Iowa, Department of Biology, Iowa City, IA, USA. This work was supported by grants from the Israel Cancer Association , the Chief Scientist's Office of the Ministry of Health, Israel , and the Israel Science Foundation (Grant No. 709/10 ).

Funders	Funder number
Israel Cancer Association
Israel Science Foundation	709/10
Ministry of Health, State of Israel

Keywords

glioma tumor suppressor candidate region 2 gene product, GLTSCR2
nucleolus
oligomer
p53
protein interacting with carboxyl terminus 1, PICT-1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jmb.2014.04.006

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title = "The nucleolar PICT-1/GLTSCR2 protein forms homo-oligomers",

abstract = "The human {"}protein interacting with carboxyl terminus 1{"} (PICT-1), also designated as the {"}glioma tumor suppressor candidate region 2 gene product{"}, GLTSCR2, is a nucleolar protein whose activity is, as yet, unknown. Contradictory results regarding the role of PICT-1 in cancer have been reported, and PICT-1 has been suggested to function either as a tumor suppressor protein or as an oncogene. In this study, we demonstrate self-association of PICT-1. Through yeast two-hybrid assay, we identified PICT-1 as its own interaction partner. We confirmed the interaction of PICT-1 with itself by direct yeast two-hybrid assay and also showed self-association of PICT-1 in mammalian cells by co-immunoprecipitation and fluorescence resonance energy transfer assays. Furthermore, we confirmed direct self-association of PICT-1 by using in vitro microfluidic affinity binding assays. The later assay also identified the carboxy-terminal domain as mediating self-interaction of PICT-1. Glutaraldehyde cross-linking and gel-filtration assays suggest that PICT-1 forms dimers, though it may form higher-order complexes as well. Our findings add another layer of complexity in understanding the different functions of PICT-1 and may help provide insights regarding the activities of this protein.",

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author = "Tatyana Borodianskiy-Shteinberg and Inna Kalt and Sarit Kipper and Nofar Nachum and Shiri Katz and Pauker, {Maor H.} and Mira Barda-Saad and Doron Gerber and Ronit Sarid",

note = "Funding Information: We gratefully acknowledge Prof. Bernard Roizman, Dr. Jordan Chill and Dr. Yarden Opatowsky for reagents, helpful discussions and technical help. Portions of this work submitted by T.B.-S. to Bar Ilan University, Ramat Gan, Israel, are in partial fulfillment of the requirements for the degree of Doctor of Philosophy. The anti-myc developed by Prof. J. M. Bishop was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by The University of Iowa, Department of Biology, Iowa City, IA, USA. This work was supported by grants from the Israel Cancer Association , the Chief Scientist's Office of the Ministry of Health, Israel , and the Israel Science Foundation (Grant No. 709/10 ). ",

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AU - Nachum, Nofar

AU - Katz, Shiri

AU - Pauker, Maor H.

AU - Barda-Saad, Mira

AU - Gerber, Doron

AU - Sarid, Ronit

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N2 - The human "protein interacting with carboxyl terminus 1" (PICT-1), also designated as the "glioma tumor suppressor candidate region 2 gene product", GLTSCR2, is a nucleolar protein whose activity is, as yet, unknown. Contradictory results regarding the role of PICT-1 in cancer have been reported, and PICT-1 has been suggested to function either as a tumor suppressor protein or as an oncogene. In this study, we demonstrate self-association of PICT-1. Through yeast two-hybrid assay, we identified PICT-1 as its own interaction partner. We confirmed the interaction of PICT-1 with itself by direct yeast two-hybrid assay and also showed self-association of PICT-1 in mammalian cells by co-immunoprecipitation and fluorescence resonance energy transfer assays. Furthermore, we confirmed direct self-association of PICT-1 by using in vitro microfluidic affinity binding assays. The later assay also identified the carboxy-terminal domain as mediating self-interaction of PICT-1. Glutaraldehyde cross-linking and gel-filtration assays suggest that PICT-1 forms dimers, though it may form higher-order complexes as well. Our findings add another layer of complexity in understanding the different functions of PICT-1 and may help provide insights regarding the activities of this protein.

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The nucleolar PICT-1/GLTSCR2 protein forms homo-oligomers

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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