Two-way and one-way mixed leukocyte reaction (MLR) was demonstrated in peripheral blood leukocytes (PBL) of carp. Primary two-way MLR in randomly selected donor pairs were highly variable. Weak primary responses could be strongly augmented by mutual in vivo priming of the reacting donors. One-way MLR was performed using irradiated (16,000 R) allogeneic PBL as stimulators. Reciprocal responses of randomly paired donors were usually unequal, suggesting the usefulness of this method for genetic analysis of MLR-recognized histocompatibility antigens in carp. Kinetics of the primary and secondary two-way MLR were studied, as well as the kinetics of primary one-way MLR against pooled allogeneic stimulator cells.
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Acknowled@ements: Supported by Research Grant NO. AQII from the National Council for Research and Development, Israel, the KGSS Geesthacht-Tesperhude, Germany, and by Grant No. 01-509 from Bar-Ilan University.