The metC gene in Escherichia coli K-12: Isolation and studies of relatedness in Enterobacteriaceae

Shulamit Michaeli; Eliora Z. Ron

doi:10.1111/j.1574-6968.1984.tb00349.x

The metC gene in Escherichia coli K-12: Isolation and studies of relatedness in Enterobacteriaceae

Shulamit Michaeli, Eliora Z. Ron

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A specialized transducing phage λ carrying the structural gene for β-cystathionase (metC) of Escherichia coli was isolated. The phage carries a 21-kb fragment of the E. coli K-12 chromosome, and its structure was analyzed using restriction enzymes. The metC gene was recloned into resistance plasmid pBR322, using EcoRI or HindIII. The information for the metC gene is contained in a 1.3-kb fragment, which shows a high degree of homology with representative strains of all tribes of Enterobacteriaceae.

Original language	English
Pages (from-to)	31-35
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	22
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1984.tb00349.x
State	Published - 1 Apr 1984
Externally published	Yes

Keywords

Methionine
cystathionase
metC

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10.1111/j.1574-6968.1984.tb00349.x

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abstract = "A specialized transducing phage λ carrying the structural gene for β-cystathionase (metC) of Escherichia coli was isolated. The phage carries a 21-kb fragment of the E. coli K-12 chromosome, and its structure was analyzed using restriction enzymes. The metC gene was recloned into resistance plasmid pBR322, using EcoRI or HindIII. The information for the metC gene is contained in a 1.3-kb fragment, which shows a high degree of homology with representative strains of all tribes of Enterobacteriaceae.",

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AB - A specialized transducing phage λ carrying the structural gene for β-cystathionase (metC) of Escherichia coli was isolated. The phage carries a 21-kb fragment of the E. coli K-12 chromosome, and its structure was analyzed using restriction enzymes. The metC gene was recloned into resistance plasmid pBR322, using EcoRI or HindIII. The information for the metC gene is contained in a 1.3-kb fragment, which shows a high degree of homology with representative strains of all tribes of Enterobacteriaceae.

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The metC gene in Escherichia coli K-12: Isolation and studies of relatedness in Enterobacteriaceae

Abstract

Keywords

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