TY - JOUR
T1 - The mesenchyme expresses T cell receptor mRNAs
T2 - Relevance to cell growth control
AU - Barda-Saad, Mira
AU - Shav-Tal, Yaron
AU - Rozenszajn, Arie Leon
AU - Cohen, Michal
AU - Zauberman, Ayelet
AU - Karmazyn, Asaf
AU - Parameswaran, Reshmi
AU - Schori, Hadas
AU - Ashush, Hagit
AU - Ben-Nun, Avraham
AU - Zipori, Dov
PY - 2002/3/27
Y1 - 2002/3/27
N2 - The mesenchyme plays a crucial regulatory role in organ formation and maintenance. However, comprehensive molecular characterization of these cells is lacking. We found unexpectedly that primary mesenchyme, as well as mesenchymal cell clones, express T cell receptor (TCR)αβ mRNAs, lacking the variable region. Immunological and genetic evidence support the expression of a corresponding TCRβ protein. Additionally, mRNAs encoding TCR complex components including CD3 and ζ chain are present. A relatively higher expression of the mesenchymal TCRβ mRNA by cultured mesenchymal cell clones correlates with fast growth, whereas poorly expressing cells are slow growers and are contact inhibited. The clones that express relatively higher amount of the TCR mRNA exhibit an increased capacity to form tumors in nude mice. However, the expression of this mRNA in the mesenchyme is not per se leading to tumorigenesis, as demonstrated by primary mesenchyme that does not form tumors in mice while expressing moderate amounts of the TCR transcripts. The expression of mesencymal TCRβ was confined to the G2/M phases of the cell cycle in the MBA-13 mesenchymal cell line. This cell cycle dependent expression, considered together with the correlation between growth properties and the level of TCR expression by cell clones, implies association of mesenchymal TCR with cell growth control.
AB - The mesenchyme plays a crucial regulatory role in organ formation and maintenance. However, comprehensive molecular characterization of these cells is lacking. We found unexpectedly that primary mesenchyme, as well as mesenchymal cell clones, express T cell receptor (TCR)αβ mRNAs, lacking the variable region. Immunological and genetic evidence support the expression of a corresponding TCRβ protein. Additionally, mRNAs encoding TCR complex components including CD3 and ζ chain are present. A relatively higher expression of the mesenchymal TCRβ mRNA by cultured mesenchymal cell clones correlates with fast growth, whereas poorly expressing cells are slow growers and are contact inhibited. The clones that express relatively higher amount of the TCR mRNA exhibit an increased capacity to form tumors in nude mice. However, the expression of this mRNA in the mesenchyme is not per se leading to tumorigenesis, as demonstrated by primary mesenchyme that does not form tumors in mice while expressing moderate amounts of the TCR transcripts. The expression of mesencymal TCRβ was confined to the G2/M phases of the cell cycle in the MBA-13 mesenchymal cell line. This cell cycle dependent expression, considered together with the correlation between growth properties and the level of TCR expression by cell clones, implies association of mesenchymal TCR with cell growth control.
KW - Cell growth
KW - Mesenchyme
KW - Stroma
KW - TCR
KW - Tumors
UR - http://www.scopus.com/inward/record.url?scp=85047697401&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1205269
DO - 10.1038/sj.onc.1205269
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C2 - 11960375
AN - SCOPUS:85047697401
SN - 0950-9232
VL - 21
SP - 2029
EP - 2036
JO - Oncogene
JF - Oncogene
IS - 13
ER -