The Mdm2 oncoprotein interacts with the cell fate regulator numb

Tamar Juven-Gershon, Ohad Shifman, Tamar Unger, Adi Elkeles, Ygal Haupt, Moshe Oren

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

The Mdm2 oncoprotein is a well-known inhibitor of the p53 tumor suppressor, but it may also possess p53-independent activities. In search of such p53-independent activities, the yeast two-hybrid screen was employed to identify Mdm2-binding proteins. We report that in vitro and in transfected cells, Mdm2 can associate with Numb, a protein involved in the determination of cell fate. This association causes translocation of overexpressed Numb into the nucleus and leads to a reduction in overall cellular Numb levels. Through its interaction with Numb, Mdm2 may influence processes such as differentiation and survival. This could potentially contribute to the altered properties of tumor cells which overexpress Mdm2.

Original languageEnglish
Pages (from-to)3974-3982
Number of pages9
JournalMolecular and Cellular Biology
Volume18
Issue number7
DOIs
StatePublished - Jul 1998
Externally publishedYes

Funding

FundersFunder number
National Cancer InstituteR01CA040099

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