The lpf operon of invasive Escherichia coli

Diana Ideses; Dvora Biran; Uri Gophna; Orlev Levy-Nissenbaum; Eliora Z. Ron

doi:10.1016/j.ijmm.2005.04.009

The lpf operon of invasive Escherichia coli

Diana Ideses, Dvora Biran, Uri Gophna, Orlev Levy-Nissenbaum, Eliora Z. Ron

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) strains have been shown to code for several virulence factors involved in adherence to host tissues. Here we show the existence of an additional adherence gene cluster, coding for long polar fimbriae - LPF - in several strains of serotype O78 from septicemia and newborn meningitis. The complete gene cluster was sequenced in strain 789 (lpf₇₈₉), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD. The lpf operon is expressed and is important for adherence to epithelial cells. The lpf operon was found only in four of the ExPEC strains tested and is likely to have been acquired by horizontal gene transfer.

Original language	English
Pages (from-to)	227-236
Number of pages	10
Journal	International Journal of Medical Microbiology
Volume	295
Issue number	4
DOIs	https://doi.org/10.1016/j.ijmm.2005.04.009
State	Published - 22 Aug 2005
Externally published	Yes

Bibliographical note

Funding Information:
The authors wish to thank Tslil Ophir for help with automated sequencing. D. Ideses thanks the Constantiner Institute for Molecular Genetics. U. Gophna is supported by a Killam postdoctoral fellowship. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.

Funding

The authors wish to thank Tslil Ophir for help with automated sequencing. D. Ideses thanks the Constantiner Institute for Molecular Genetics. U. Gophna is supported by a Killam postdoctoral fellowship. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.

Funders	Funder number
Israel Center for Emerging Diseases
Manja and Morris Leigh Chair for Biophysics and Biotechnology
European Commission

Keywords

Adherence
Avian colisepticemia
Extraintestinal E. coli
Newborn meningitis
PhoA gene fusion
Serotype O78

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijmm.2005.04.009

Cite this

@article{1f1d17859e8a4deea16834b3f86cde19,

title = "The lpf operon of invasive Escherichia coli",

abstract = "Extraintestinal pathogenic Escherichia coli (ExPEC) strains have been shown to code for several virulence factors involved in adherence to host tissues. Here we show the existence of an additional adherence gene cluster, coding for long polar fimbriae - LPF - in several strains of serotype O78 from septicemia and newborn meningitis. The complete gene cluster was sequenced in strain 789 (lpf789), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD. The lpf operon is expressed and is important for adherence to epithelial cells. The lpf operon was found only in four of the ExPEC strains tested and is likely to have been acquired by horizontal gene transfer.",

keywords = "Adherence, Avian colisepticemia, Extraintestinal E. coli, Newborn meningitis, PhoA gene fusion, Serotype O78",

author = "Diana Ideses and Dvora Biran and Uri Gophna and Orlev Levy-Nissenbaum and Ron, {Eliora Z.}",

note = "Funding Information: The authors wish to thank Tslil Ophir for help with automated sequencing. D. Ideses thanks the Constantiner Institute for Molecular Genetics. U. Gophna is supported by a Killam postdoctoral fellowship. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.",

year = "2005",

month = aug,

day = "22",

doi = "10.1016/j.ijmm.2005.04.009",

language = "אנגלית",

volume = "295",

pages = "227--236",

journal = "International Journal of Medical Microbiology",

issn = "1438-4221",

publisher = "Elsevier GmbH",

number = "4",

}

TY - JOUR

T1 - The lpf operon of invasive Escherichia coli

AU - Ideses, Diana

AU - Biran, Dvora

AU - Gophna, Uri

AU - Levy-Nissenbaum, Orlev

AU - Ron, Eliora Z.

N1 - Funding Information: The authors wish to thank Tslil Ophir for help with automated sequencing. D. Ideses thanks the Constantiner Institute for Molecular Genetics. U. Gophna is supported by a Killam postdoctoral fellowship. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.

PY - 2005/8/22

Y1 - 2005/8/22

N2 - Extraintestinal pathogenic Escherichia coli (ExPEC) strains have been shown to code for several virulence factors involved in adherence to host tissues. Here we show the existence of an additional adherence gene cluster, coding for long polar fimbriae - LPF - in several strains of serotype O78 from septicemia and newborn meningitis. The complete gene cluster was sequenced in strain 789 (lpf789), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD. The lpf operon is expressed and is important for adherence to epithelial cells. The lpf operon was found only in four of the ExPEC strains tested and is likely to have been acquired by horizontal gene transfer.

AB - Extraintestinal pathogenic Escherichia coli (ExPEC) strains have been shown to code for several virulence factors involved in adherence to host tissues. Here we show the existence of an additional adherence gene cluster, coding for long polar fimbriae - LPF - in several strains of serotype O78 from septicemia and newborn meningitis. The complete gene cluster was sequenced in strain 789 (lpf789), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD. The lpf operon is expressed and is important for adherence to epithelial cells. The lpf operon was found only in four of the ExPEC strains tested and is likely to have been acquired by horizontal gene transfer.

KW - Adherence

KW - Avian colisepticemia

KW - Extraintestinal E. coli

KW - Newborn meningitis

KW - PhoA gene fusion

KW - Serotype O78

UR - http://www.scopus.com/inward/record.url?scp=22544446175&partnerID=8YFLogxK

U2 - 10.1016/j.ijmm.2005.04.009

DO - 10.1016/j.ijmm.2005.04.009

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 16128397

AN - SCOPUS:22544446175

SN - 1438-4221

VL - 295

SP - 227

EP - 236

JO - International Journal of Medical Microbiology

JF - International Journal of Medical Microbiology

IS - 4

ER -

The lpf operon of invasive Escherichia coli

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this