The long-range electrostatic interactions control tRNA-aminoacyl-tRNA synthetase complex formation

Dmitry Tworowski, Mark Safro

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

In most cases aminoacyl-tRNA synthetases (aaRSs) are negatively charged, as are the tRNA substrates. It is apparent that there are driving forces that provide a long-range attraction between like charge aaRS and tRNA, and ensure formation of "close encounters." Based on numerical solutions to the nonlinear Poisson-Boltzmann equation, we evaluated the electrostatic potential generated by different aaRSs. The 3D-isopotential surfaces calculated for different aaRSs at 0.01 kT/e contour level reveal the presence of large positive patches - one patch for each tRNA molecule. This is true for classes I and II monomers, dimers, and heterotetramers. The potential maps keep their characteristic features over a wide range of contour levels. The results suggest that nonspecific electrostatic interactions are the driving forces of primary stickiness of aaRSs-tRNA complexes. The long-range attraction in aaRS-tRNA systems is explained by capture of negatively charged tRNA into "blue space area" of the positive potential generated by aaRSs. Localization of tRNA in this area is a prerequisite for overcoming the barrier of Brownian motion.

Original languageEnglish
Pages (from-to)1247-1251
Number of pages5
JournalProtein Science
Volume12
Issue number6
DOIs
StatePublished - 1 Jun 2003
Externally publishedYes

Keywords

  • Aminoacyl-tRNA synthetase
  • Brownian motion
  • Electrostatic interactions
  • Electrostatic potential
  • Encounter complex
  • tRNA

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