The leader peptide of MMTV Env precursor localizes to the nucleoli in MMTV-derived T cell lymphomas and interacts with nucleolar protein B23

Hagit Hoch-Marchaim; Aryeh M. Weiss; Allan Bar-Sinai; Menachem Fromer; Knut Adermann; Jacob Hochman

doi:10.1016/S0042-6822(03)00236-8

The leader peptide of MMTV Env precursor localizes to the nucleoli in MMTV-derived T cell lymphomas and interacts with nucleolar protein B23

Hagit Hoch-Marchaim, Aryeh M. Weiss, Allan Bar-Sinai, Menachem Fromer, Knut Adermann, Jacob Hochman

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We have previously described two nucleolar proteins, named p14 and p21, in MMTV-induced T cell lymphomas. These proteins were identified by a monoclonal antibody (M-66) generated from a nontumorigenic, immunogenic variant of S49 T cell lymphoma. While p14 was common to several MMTV-derived T cell lymphomas, p21 was found only in highly tumorigenic variants of S49 cells. Here we report that p14 is the leader peptide of the MMTV env precursor. The epitope recognized by M-66 contains a putative nuclear localization signal. Actinomycin D was found to induce redistribution of p14/p21 from the nucleolus to the nucleoplasm. p14 coimmunoprecipitated and colocalized with the cellular protein, B23. Association with B23 has been previously reported for other auxiliary nucleolar retroviral proteins, such as Rev (HIV) and Rex (HTLV).

Original language	English
Pages (from-to)	22-32
Number of pages	11
Journal	Virology
Volume	313
Issue number	1
DOIs	https://doi.org/10.1016/S0042-6822(03)00236-8
State	Published - 15 Aug 2003
Externally published	Yes

Bibliographical note

Funding Information:
We thank Dr. Aryeh Admon’s laboratory, Protein Research Center, Technion, Haifa, for the amino acid sequence determination. This study was supported in part by the Israel Science Foundation (J.H.) and by the Niedersachsen Ministry of Science (K.A. and J.H.).

Funding

We thank Dr. Aryeh Admon’s laboratory, Protein Research Center, Technion, Haifa, for the amino acid sequence determination. This study was supported in part by the Israel Science Foundation (J.H.) and by the Niedersachsen Ministry of Science (K.A. and J.H.).

Funders	Funder number
Niedersachsen Ministry of Science
Israel Science Foundation

Keywords

MMTV
Murine lymphoma
Nucleolus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0042-6822(03)00236-8

Cite this

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title = "The leader peptide of MMTV Env precursor localizes to the nucleoli in MMTV-derived T cell lymphomas and interacts with nucleolar protein B23",

abstract = "We have previously described two nucleolar proteins, named p14 and p21, in MMTV-induced T cell lymphomas. These proteins were identified by a monoclonal antibody (M-66) generated from a nontumorigenic, immunogenic variant of S49 T cell lymphoma. While p14 was common to several MMTV-derived T cell lymphomas, p21 was found only in highly tumorigenic variants of S49 cells. Here we report that p14 is the leader peptide of the MMTV env precursor. The epitope recognized by M-66 contains a putative nuclear localization signal. Actinomycin D was found to induce redistribution of p14/p21 from the nucleolus to the nucleoplasm. p14 coimmunoprecipitated and colocalized with the cellular protein, B23. Association with B23 has been previously reported for other auxiliary nucleolar retroviral proteins, such as Rev (HIV) and Rex (HTLV).",

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AU - Bar-Sinai, Allan

AU - Fromer, Menachem

AU - Adermann, Knut

AU - Hochman, Jacob

N1 - Funding Information: We thank Dr. Aryeh Admon’s laboratory, Protein Research Center, Technion, Haifa, for the amino acid sequence determination. This study was supported in part by the Israel Science Foundation (J.H.) and by the Niedersachsen Ministry of Science (K.A. and J.H.).

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N2 - We have previously described two nucleolar proteins, named p14 and p21, in MMTV-induced T cell lymphomas. These proteins were identified by a monoclonal antibody (M-66) generated from a nontumorigenic, immunogenic variant of S49 T cell lymphoma. While p14 was common to several MMTV-derived T cell lymphomas, p21 was found only in highly tumorigenic variants of S49 cells. Here we report that p14 is the leader peptide of the MMTV env precursor. The epitope recognized by M-66 contains a putative nuclear localization signal. Actinomycin D was found to induce redistribution of p14/p21 from the nucleolus to the nucleoplasm. p14 coimmunoprecipitated and colocalized with the cellular protein, B23. Association with B23 has been previously reported for other auxiliary nucleolar retroviral proteins, such as Rev (HIV) and Rex (HTLV).

AB - We have previously described two nucleolar proteins, named p14 and p21, in MMTV-induced T cell lymphomas. These proteins were identified by a monoclonal antibody (M-66) generated from a nontumorigenic, immunogenic variant of S49 T cell lymphoma. While p14 was common to several MMTV-derived T cell lymphomas, p21 was found only in highly tumorigenic variants of S49 cells. Here we report that p14 is the leader peptide of the MMTV env precursor. The epitope recognized by M-66 contains a putative nuclear localization signal. Actinomycin D was found to induce redistribution of p14/p21 from the nucleolus to the nucleoplasm. p14 coimmunoprecipitated and colocalized with the cellular protein, B23. Association with B23 has been previously reported for other auxiliary nucleolar retroviral proteins, such as Rev (HIV) and Rex (HTLV).

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The leader peptide of MMTV Env precursor localizes to the nucleoli in MMTV-derived T cell lymphomas and interacts with nucleolar protein B23

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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